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ZIRC
ZFIN ID: ZDB-FIG-141218-4
Lai et al., 2014 - Zebrafish WNK Lysine Deficient Protein Kinase 1 (wnk1) Affects Angiogenesis Associated with VEGF Signaling. PLoS One   9:e106129 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 5

Effect of wnk1a or wnk1b knockdown on vasculogenesis and angiogenesis in Tg(fli1:GFP) embryos.

(A, B) Whole mount in situ hybridization for etv2 at 14 and 18 hpf in uninjected control embryos (A1, B1), wnk1a morphants (A2, B2), and wnk1b morphants (A3, B3). Flat mounts of de-yolked embryos were prepared. (C) GFP fluorescence was used to assay ISV formation in uninjected control embryos (C1), wnk1a morphants (C2), and wnk1b morphants (C3) at 33 hpf. (D1) etv2 expression in uninjected control, wnk1a and wnk1b morphants as a percentage of embryos exhibit strong or expression, (D2) ISV length in wnk1a and wnk1b morphants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg standard conditions Prim-15 cranial vasculature IFL
Prim-15 intersegmental vessel IFL
Prim-15 trunk vasculature IFL
y1Tg + MO1-wnk1a standard conditions Prim-15 cranial vasculature IFL
Prim-15 intersegmental vessel IFL
Prim-15 trunk vasculature IFL
y1Tg + MO4-wnk1b standard conditions Prim-15 cranial vasculature IFL
Prim-15 intersegmental vessel IFL
Prim-15 trunk vasculature IFL
etsrp WT standard conditions 10-13 somites blood vessel endothelial cell ISH
14-19 somites blood vessel endothelial cell ISH
WT + MO1-wnk1a standard conditions 10-13 somites blood vessel endothelial cell ISH
14-19 somites blood vessel endothelial cell ISH
WT + MO4-wnk1b standard conditions 10-13 somites blood vessel endothelial cell ISH
14-19 somites blood vessel endothelial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-wnk1a standard conditions Prim-15 intersegmental vein decreased length, abnormal
Prim-15 intersegmental vessel angiogenesis process quality, abnormal
y1Tg + MO4-wnk1b standard conditions Prim-15 intersegmental vein decreased length, abnormal
Prim-15 intersegmental vessel angiogenesis process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One