kremen1, dkk1b and dkk2 are expressed in the pLLP of WT and krm1nl10 mutant embryos. (A-L) Expression of kremen1, dkk1b and dkk2 in the primordia (highlighted by dashed lines) of WT and krm1nl10 embryos at 32 and 40hpf. kremen1 expression is progressively decreased and restricted to the leading zone between 32 and 40hpf in WT (A,C) and krm1nl10 (B,D) embryos. dkk1b is expressed in the mid-zone of the pLLP at 32 and 40hpf in WT (E,G) and krm1nl10 (F,H) embryos. dkk2 is expressed in the mid-zone at 32hpf in WT (I) and mutant (J) primordia. At 40hpf, dkk2 is expressed in the mid-zone of WT pLLP (K) and in the last deposited NM in mutant embryos; faint expression is present throughout the mutant pLLP (L). Scale bar: 20μm.