Fig. 4

Li et al., 2014 - Zebrafish Adar2 Edits the Q/R Site of AMPA Receptor Subunit gria2alpha Transcript to Ensure Normal Development of Nervous System and Cranial Neural Crest Cells
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Fig. 4

Increased p53-dependent apoptosis in the hypo-Q/R-editing morphants.

(A) Apoptosis at the 5-somite stage. Representative images, except those co-injected with p53-MOAUG, were selected from the ones showing the mean fluorescence intensities in the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) analysis. The anterior is to the left, and dorsal side is to the top. Top panel shows the adar2MOc, adar2MOsp and adar2MOt. Middle panel shows adar2MOt co-injected with p53AUG morpholino to block p53 activity, with adar2 cRNA (Adar2) and with adar2cd cRNA (Adar2cd). The bottom panel shows gria2αQRMO, and the gria2αQRMO co-injected with p53AUG and with adar2 cRNA. (B) Tukey box plot of the relative TUNEL signals at 5-somite stage. Boxes represent 50% inter quartile values. Black and red lines respectively mark the median and mean intensities. Outliers are marked as dots. All signals were normalized to a wild-type embryo, showing mean intensity, stained in the same batch (relative fluorescence intensity). The total embryos included in the analysis are 8 adar2MOc (2 batches), 42 adar2MOt (9 batches), 19 adar2MOt-p53 AUG (3 batches), 20 adar2MOt+Adar2 (3 batches), 12 adar2MOt+Adar2cd (3 batches), 9 gria2αQRMO (3 batches), 7 gria2αQRMO-p53AUG (3 batches) and 5 gria2αQRMO+ Adar2 (2 batches). (C) Increased p53-dependent apoptosis in specific regions of the 24- and 36-hpf hypo-Q/R-editing morphants. Lateral views of head and trunk at 24 and 36 hpf. Arrows indicate the apoptotic cells along the horizontal myoseptum. Scale bars represent 100 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to Prim-25

Phenotype Detail
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