Fig. 2

Zebrafish cx27.5 and cx30.3 transcripts expressed during embryo development stage and in adult tissues. (A) The expression of the zebrafish cx27.5 and cx30.3 gene during embryonic stage. Total RNAs from various stages of zebrafish were used as template for RT-PCR. The cx27.5 gene expressed at low levels in the 1.5 to 3 hpf and dominantly expressed after 6-96 hpf. In contrast, cx30.3 transcripts were hardly detected until 9 hpf. (B) Total RNAs from various organs of zebrafish were conducted to use as template for RT-PCR. Each bar shows the expression of cx27.5 or cx30.3 transcripts in various tissues of adult zebrafish relative to the expression of zebrafish elongation factor 1-alpha (ef1α). The ef1α was used as an internal control. (C) We found that the cx27.5 gene expressed in most adult tissues, except liver and gall bladder tissues. Meanwhile, the cx30.3 gene dominantly expressed in the cells of ovary, skin and inner ear of zebrafish. hpf: hour post fertilization, N: negative control.