ZFIN ID: ZDB-FIG-140618-18
Quillien et al., 2014 - Distinct Notch signaling outputs pattern the developing arterial system. Development (Cambridge, England)   141:1544-52 Full text @ Development
Anatomical Terms:
Stage Range: 5-9 somites to 10-13 somites

Fig. 1

Notch signaling is active in early endothelial progenitors. (A-E) Confocal micrographs of flat-mounted Tg(tp1:egfp)um14 embryos following fluorescence in situ hybridization using riboprobes against egfp (green) and etv2 (red). (A,C-E) Dorsal views. (A-C) Images from the same embryo at 5 ss. (A,D) tp1:egfp expression; (A2,D2) tp1:egfp plus etv2 expression. (A) Arrows indicate neural crest (nc), proneural clusters (pnc); bracket denotes somitic mesoderm (sm). (B) Lateral view of embryo in A showing egfp expression in neural crest (white arrow), dorsal to etv2-expressing cells. The yellow arrow denotes the egfp/etv2-positive cell shown in C-C2 white arrowheads indicate the etv2-positive/egfp-negative cells. (C) Magnified area indicated by box in A2. (D,E) Images of the same embryo at 12 ss. (D2) Box indicates area magnified in E. (E) White arrowheads indicate etv2-positive/egfp-negative cells; yellow arrows denote egfp/etv2-coexpression. Scale bars: 100 μm (A,B,D); 40 μm (C,E).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP um14Tg standard conditions 5-9 somites neural crest ISH
5-9 somites proneural cluster ISH
5-9 somites segmental plate ISH
5-9 somites somite ISH
10-13 somites endothelial cell ISH
10-13 somites segmental plate ISH
etsrp um14Tg standard conditions 5-9 somites endothelial cell ISH
10-13 somites endothelial cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development