ZFIN ID: ZDB-FIG-140425-4
Raymond et al., 2014 - Patterning the cone mosaic array in zebrafish retina requires specification of ultraviolet-sensitive cones. PLoS One   9(1):e85325 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 5

UV cones in tbx2b mutants are dysmorphic.

A) Retinal cryosection from a sws1:EGFP adult zebrafish; UV cones are magenta, red-green double cones are immunostained with the specific marker, zpr1 (yellow), and nuclei are stained with the fluorescent dye, Hoechst (grey). The central panel is an overlay of the epifluorescent channel (UV cones) and transmitted light (differential interference contrast). Nuclei of red-green double cones (arrows), and blue cones (not indicated), are radially elongated and displaced apical to the outer limiting membrane (dashed line), whereas nuclei of UV cones are triangular in shape (circles) and positioned basal to the outer limiting membrane. B, C) In tbx2b; sws1:EGFP mutants, the morphology of red-green double cones (zpr1, yellow) and their nuclear position (white arrows) is normal, whereas the nuclei of UV cones (circles) are displaced apically beyond the outer limiting membrane (dashed line). One UV cone (black arrow) is collapsed. D, E) 3D volume renderings of UV cones near the retinal margin in wild-type, sws1:EGFP fish. Like all photoreceptors UV cones have a single, bulbous axonal terminal (cone pedicle, arrow). D) Many UV cones in tbx2b; sws1:EGFP mutant adults have a bifurcated axon and two pedicles (arrows). Scale bar: 20 μm (A,B,C).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP kj9Tg standard conditions Adult UV sensitive photoreceptor cell IHC
tbx2bc144/c144; kj9Tg standard conditions Adult UV sensitive photoreceptor cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC kj9Tg standard conditions Adult green sensitive photoreceptor cell
IHC Adult red sensitive photoreceptor cell
IHC tbx2bc144/c144; kj9Tg standard conditions Adult green sensitive photoreceptor cell
IHC Adult red sensitive photoreceptor cell
Phenotype Details
Fish Conditions Stage Phenotype
tbx2bc144/c144; kj9Tg standard conditions Adult UV sensitive photoreceptor cell axon bifurcated, abnormal
Adult UV sensitive photoreceptor cell deformed, abnormal
Adult UV sensitive photoreceptor cell has extra parts of type UV sensitive photoreceptor cell cone cell pedicle, abnormal
Adult UV sensitive photoreceptor cell morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One