ZFIN ID: ZDB-FIG-140425-1
Raymond et al., 2014 - Patterning the cone mosaic array in zebrafish retina requires specification of ultraviolet-sensitive cones. PLoS One   9(1):e85325 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 1

Rod photoreceptors develop rapidly and UV cones are missing in tbx2b mutants.

A) Isolated, larval rh1:EGFP eye at 3 days post-fertilization (dpf) viewed from the scleral aspect. Rod photoreceptors are green and immunostaining for the apical junctional marker, Zonula Occludens-1 (ZO-1) is in white. Dorsal is up; the optic nerve appears as a small white ring ventral to the center. B) Mutant tbx2b; rh1:EGFP larval eye at 3 dpf viewed from the scleral side. Note increased number of rods, especially in the ventral retina. C, D) Higher magnifications of central retina in wild-type and mutant eyes, respectively. E) Isolated, larval sws1:EGFP eye and F) tbx2b; sws1:EGFP eye at 4 dpf, viewed from the scleral aspect. Cones expressing the UV opsin reporter are pseudocolored magenta. The lens, outlined with a dashed white line, shows background fluorescence in F, due to the longer exposure time required to capture immunofluorescence of the few scattered UV cones. Scale bars: = 50 μm (A,B); 10 μm (C,D); 50 μm (E,F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP kj2Tg standard conditions Protruding-mouth retinal rod cell IHC
kj9Tg standard conditions Day 4 UV sensitive photoreceptor cell IHC
tbx2bc144/c144; kj2Tg standard conditions Protruding-mouth retinal rod cell IHC
tbx2bc144/c144; kj9Tg standard conditions Day 4 UV sensitive photoreceptor cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC kj2Tg standard conditions Protruding-mouth outer limiting membrane
IHC tbx2bc144/c144; kj2Tg standard conditions Protruding-mouth outer limiting membrane
Phenotype Details
Fish Conditions Stage Phenotype
tbx2bc144/c144; kj2Tg standard conditions Protruding-mouth retina has extra parts of type retinal rod cell, abnormal
tbx2bc144/c144; kj9Tg standard conditions Day 4 retina has fewer parts of type UV sensitive photoreceptor cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One