ZFIN ID: ZDB-FIG-140325-49
Wylie et al., 2014 - Post-transcriptional regulation of wnt8a is essential to zebrafish axis development. Developmental Biology   386(1):53-63 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Shield
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 6

UTR2 TP MO elevates wnt8a levels. (A–F) In situ hybridizations, 60–80% epiboly. Probe indicated in upper right, relative probe position is shown in Fig. 1. (A, C, and E) Control morpholino injected. (B,D,F) UTR2 TP MO injected. Note increased staining for wnt8a.1 and wnt8a.2. Insets in A and B: Close-up comparison to highlight differences in wnt8a.1. (G) RT-qPCR quantification of expression changes. Y-axis indicates fold change from levels in control injected embryos. Box indicates ±standard deviation, line in box represents mean. Asterisks indicate p<0.01. (H) Lateral views of live 24 hpf embryos. Treatment indicated above each image. (I) Percent of embryos per class after morpholino co-injection. Number in parentheses=n for each treatment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lef1 WT standard conditions Shield margin ISH
WT + MO7-wnt8a standard conditions Shield margin ISH
wnt8a WT standard conditions Shield margin ISH
WT + MO7-wnt8a standard conditions Shield margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-wnt8a + MO2-wnt8a standard conditions Prim-5 whole organism wholly dorsalized, abnormal
WT + MO1-wnt8a + MO2-wnt8a + MO7-wnt8a standard conditions Prim-5 whole organism dead, abnormal
Prim-5 whole organism wholly dorsalized, abnormal
WT + MO7-wnt8a standard conditions Prim-5 extension increased size, abnormal
Prim-5 eye decreased size, abnormal
Prim-5 head malformed, abnormal
Prim-5 whole organism dead, abnormal
Prim-5 whole organism wholly ventralized, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 386(1), Wylie, A.D., Fleming, J.A., Whitener, A.E., and Lekven, A.C., Post-transcriptional regulation of wnt8a is essential to zebrafish axis development, 53-63, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.