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ZFIN ID: ZDB-FIG-140325-1
Shiau et al., 2013 - An Anti-inflammatory NOD-like Receptor Is Required for Microglia Development. Cell Reports   5(5):1342-1352 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1

Lack of Microglia in nlrc3-like Mutants

(A and B) Live 5 dpf zebrafish larvae were stained by neutral red to visualize microglia in the brain.

(A) Heterozygous st73/+ larva (arrow points to microglia around optic tectum) is shown.

(B) Homozygous st73 larvae have no microglia (arrow). Dorsal views; anterior is to the top.

(C and D) Microglial marker apoe expression at 5 dpf in the brain is shown.

(E and F) Neutral red-stained heterozygous (E) and homozygous mutant (F) shows normal whole-animal morphologies at 5 dpf. Lateral views; anterior is to the left.

(G) Genetic and physical map of the st73 locus.

(H) Sequence chromatogram shows the lesion (asterisks) in the coding sequence of nlrc3-like (LOC100538217).

(I) The st73 mutation introduces a premature stop codon near the 52 end of the open reading frame of nlrc3-like.

See also Figure S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
apoeb nlrc3lst73/+ standard conditions Day 5 brain microglial cell ISH
nlrc3lst73/st73 standard conditions Day 5 Not Detected brain microglial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nlrc3lst73/+ standard conditions Day 5 brain has normal numbers of parts of type microglial cell, normal
nlrc3lst73/st73 standard conditions Day 5 brain lacks all parts of type microglial cell, abnormal
nlrc3lst73/st73 standard conditions Day 5 microglia development decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.