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ZIRC
ZFIN ID: ZDB-FIG-140324-15
Wang et al., 2013 - Impact of a combined high cholesterol diet and high glucose environment on vasculature. PLoS One   8(12):e81485 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Days 14-20
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Days 14-20

Fig. 2

Endothelial layer thickening of optical vessels in HCD-HG treatment group.

A: Different treatments led to changes of endothelial layer in optical arteries. Transgenic zebrafish (Fli1:EGFP) larvae were imaged in a lateral position by confocal microscope, and emission wavelength of 488 nm was detected. Scale bar = 10 μm. B: Endothelial layer thickness (T) was measured by subtracting inner diameter (Di) from outer diameter (Do), T = (Do-Di)/2. Changes in the thickness of endothelial layer were quantified and presented as mean ±SD. Scale bar = 20 μm. C: Pioglitazone and metformin effectively prevented the endothelial layer from becoming thick afer HCD-HG treatment. N = 11 in the control group, N = 24 in the HCD-HG group, N = 10 in HCD-HG+ pioglitazone group, N = 18 in HCD-HG+ metformin group. PGZ: pioglitazone; Met: metformin. Asterisk (*): Comparison of EC layer thickness between control group and HCD-HG group (p<0.05). Cross (†): Comparison of EC layer thickness between control and drugs treatment groups (p<0.05).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Days 14-20 optic artery vascular endothelium IFL
y1Tg high cholesterol, high glucose Days 14-20 optic artery vascular endothelium IFL
y1Tg high cholesterol, high glucose, chemical treatment: metformin Days 14-20 optic artery vascular endothelium IFL
y1Tg high cholesterol, high glucose, chemical treatment: pioglitazone Days 14-20 optic artery vascular endothelium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg high cholesterol, high glucose Days 14-20 optic artery vascular endothelium increased thickness, abnormal
y1Tg high cholesterol, high glucose, chemical treatment: metformin Days 14-20 optic artery vascular endothelium thickness, ameliorated
y1Tg high cholesterol, high glucose, chemical treatment: pioglitazone Days 14-20 optic artery vascular endothelium thickness, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One