ZFIN ID: ZDB-FIG-140305-52
Thakur et al., 2014 - Dysregulated phosphatidylinositol signaling promotes endoplasmic reticulum stress-mediated intestinal mucosal injury and inflammation in zebrafish. Disease models & mechanisms   7(1):93-106 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Day 6 to Days 7-13

Fig. S1

The cdiptlop (lop) and cdipthi559 (hi559) mutants exhibit identical GI phenotype and fail to rescue each other in complementation assay. (A) Lateral view of ISH of intestine-specific markers vil1 and anxa2b showing reduced expression in the hi559 intestine at 5-dpf. (B-F) Morphology of intestine shown by bright-field images (left panel) and fluorescent images (right panel, Nile-Red staining shown by white outline). (B) GI morphology of hi559 larvae at 6-dpf shows smaller intestine and lumen. (C) GI morphology of lop larvae shows smaller intestine and lumen at 8-dpf comparable to the 6-dpf hi559 larvae. (D) Cdipthi559/lop (hi559 X lop) trans-heterozygote mutant larvae show smaller intestinal lumen at 7.5-dpf comparable to the 6- dpf hi559 larvae. (E) Wild-type embryos were microinjected with either control morpholino or splice-blocking morpholino against cdipt at 1-cell stage and larvae were analyzed at 6-dpf (Thakur et al., 2011). Cdipt morphants show smaller intestinal structures replicating hi559 phenotype. (F) Wild-type larvae were treated with PI synthase inhibitor δ-HCH shows smaller intestinal structures compared to DMSO treated larvae.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
anxa2b WT standard conditions Day 5 intestine ISH
cdipthi559Tg/hi559Tg standard conditions Day 5 intestine ISH
vil1 WT standard conditions Day 5 intestine ISH
cdipthi559Tg/hi559Tg standard conditions Day 5 intestine ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: delta-hexachlorocyclohexane Day 6 intestine decreased size, abnormal
Day 6 intestine decreased volume, abnormal
WT + MO5-cdipt standard conditions Day 6 intestine decreased size, abnormal
Day 6 intestine decreased volume, abnormal
cdipthi559Tg/hi559Tg standard conditions Day 6 intestine decreased size, abnormal
Day 6 intestine decreased volume, abnormal
cdiptnt9/+; cdipthi559Tg/+ standard conditions Days 7-13 intestine decreased size, abnormal
Days 7-13 intestine decreased volume, abnormal
cdiptnt9/nt9 standard conditions Days 7-13 intestine decreased size, abnormal
Days 7-13 intestine decreased volume, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.