ZFIN ID: ZDB-FIG-131231-38
van der Velden et al., 2013 - The polycomb group protein ring1b/rnf2 is specifically required for craniofacial development. PLoS One   8(9):e73997 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 2 Cranial musculature development is severely impaired in ring1b mutants.

Ventral views of WT and ring1b embryos stained with the MF20 antibody (A-F). The anterior mandibularis (am) has not formed in ring1b mutants at 56 hpf (B), the sternohyoideus (sh) is reduced at 65 hpf (D) and at 72 hpf, cranial muscles are almost completely absent (F). hh: hyohyoideus; ih: interhyoideus; ima: intermandibularis anterioris; imp: intermandibularis posterioris.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-MF20 IHC WT standard conditions Long-pec adductor mandibulae
IHC Pec-fin adductor mandibulae
IHC Pec-fin hyohyoideus
IHC Pec-fin interhyoideus
IHC Pec-fin sternohyoid
IHC Protruding-mouth adductor mandibulae
IHC Protruding-mouth hyohyoideus
IHC Protruding-mouth interhyoideus
IHC Protruding-mouth sternohyoid
IHC Protruding-mouth ventral intermandibularis anterior
IHC Protruding-mouth ventral intermandibularis posterior
IHC rnf2ibl30/ibl30 standard conditions Long-pec Not Detected adductor mandibulae
IHC Pec-fin Not Detected adductor mandibulae
IHC Pec-fin Not Detected hyohyoideus
IHC Pec-fin Not Detected interhyoideus
IHC Pec-fin sternohyoid
IHC Protruding-mouth Not Detected adductor mandibulae
IHC Protruding-mouth Not Detected hyohyoideus
IHC Protruding-mouth Not Detected interhyoideus
IHC Protruding-mouth sternohyoid
IHC Protruding-mouth Not Detected ventral intermandibularis anterior
IHC Protruding-mouth Not Detected ventral intermandibularis posterior
IHC rnf2ibl31/ibl31 standard conditions Long-pec Not Detected adductor mandibulae
IHC Pec-fin Not Detected adductor mandibulae
IHC Pec-fin Not Detected hyohyoideus
IHC Pec-fin Not Detected interhyoideus
IHC Pec-fin sternohyoid
IHC Protruding-mouth Not Detected adductor mandibulae
IHC Protruding-mouth Not Detected hyohyoideus
IHC Protruding-mouth Not Detected interhyoideus
IHC Protruding-mouth sternohyoid
IHC Protruding-mouth Not Detected ventral intermandibularis anterior
IHC Protruding-mouth Not Detected ventral intermandibularis posterior
Phenotype Details
Fish Conditions Stage Phenotype
rnf2ibl30/ibl30 standard conditions Long-pec branchiomeric skeletal muscle development decreased process quality, abnormal
Long-pec head lacks all parts of type adductor mandibulae, abnormal
Pec-fin branchiomeric skeletal muscle development decreased process quality, abnormal
Pec-fin head lacks all parts of type adductor mandibulae, abnormal
Pec-fin head lacks all parts of type hyohyoideus, abnormal
Pec-fin head lacks all parts of type interhyoideus, abnormal
Pec-fin head lacks all parts of type sternohyoid, abnormal
Pec-fin sternohyoid decreased size, abnormal
Protruding-mouth head lacks all parts of type adductor mandibulae, abnormal
Protruding-mouth head lacks all parts of type hyohyoideus, abnormal
Protruding-mouth head lacks all parts of type interhyoideus, abnormal
Protruding-mouth head lacks all parts of type sternohyoid, abnormal
Protruding-mouth head lacks all parts of type ventral intermandibularis anterior, abnormal
Protruding-mouth head lacks all parts of type ventral intermandibularis posterior, abnormal
Protruding-mouth sternohyoid decreased size, abnormal
rnf2ibl31/ibl31 standard conditions Long-pec branchiomeric skeletal muscle development decreased process quality, abnormal
Long-pec head lacks all parts of type adductor mandibulae, abnormal
Pec-fin branchiomeric skeletal muscle development decreased process quality, abnormal
Pec-fin head lacks all parts of type adductor mandibulae, abnormal
Pec-fin head lacks all parts of type hyohyoideus, abnormal
Pec-fin head lacks all parts of type interhyoideus, abnormal
Pec-fin head lacks all parts of type sternohyoid, abnormal
Pec-fin sternohyoid decreased size, abnormal
Protruding-mouth head lacks all parts of type adductor mandibulae, abnormal
Protruding-mouth head lacks all parts of type hyohyoideus, abnormal
Protruding-mouth head lacks all parts of type interhyoideus, abnormal
Protruding-mouth head lacks all parts of type sternohyoid, abnormal
Protruding-mouth head lacks all parts of type ventral intermandibularis anterior, abnormal
Protruding-mouth head lacks all parts of type ventral intermandibularis posterior, abnormal
Protruding-mouth sternohyoid decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One