ZFIN ID: ZDB-FIG-131218-3
Phillips et al., 2013 - The cone-dominant retina and the inner ear of zebrafish express the ortholog of CLRN1, the causative gene of human Usher syndrome type 3A. Gene Expression Patterns   13(8):473-81 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene: clrn1
Antibodies: Ab1-clrn1, Ab1-crb2a, Ab1-crb2b, Ab1-glul
Fish: AB/TU
Anatomical Terms: Muller cell, Muller cell glial cell projection, outer limiting membrane, photoreceptor inner segment layer, photoreceptor inner segment layer glial cell projection (all 6) expand
Stage: Adult

Fig. 5

Clarin-1 localizes at interfaces between photoreceptor inner segments in the adult retina. (A-C) Cross (A and B) and transverse (C) sections through adult retinas show Clarin-1 localization partially overlapping with glial cells labeled with Glutamine Synthetase (GS) at the OLM (arrowhead). Enrichment of Clarin-1 is also noted in the subapical region (SAR) and between all cone inner segments in the outer retina. The silhouettes of the four cone subtypes are outlined in white in A, D and G and the mosaic cone arrangement is labeled in C, F and I. (D-I) Cross (D-E, G-H) and transverse (F, I) sections of adult retinas labeled with Crb2a (D-F) and Crb2b (G-I) antibodies recapitulate results published by Zou et al., 2012, and provide points of reference for the Clarin-1 (A-C) localization pattern at lateral cone interfaces. Abbreviations: OLM: outer limiting membrane; G: green opsin cone, R: red opsin cone; B: blue opsin cone; SAR (subapical region) U: UV opsin cone. Scale bars: 10 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
clrn1 Ab1-clrn1 AB/TU standard conditions Adult Muller cell glial cell projection IHC
Ab1-clrn1 Adult outer limiting membrane IHC
Ab1-clrn1 Adult photoreceptor inner segment layer IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-crb2a IHC AB/TU standard conditions Adult Muller cell glial cell projection
IHC Adult photoreceptor inner segment layer
Ab1-crb2b IHC AB/TU standard conditions Adult photoreceptor inner segment layer
IHC Adult retinal cone cell photoreceptor inner segment
Ab1-glul IHC AB/TU standard conditions Adult Muller cell
IHC Adult Muller cell glial cell projection
IHC Adult photoreceptor inner segment layer glial cell projection
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Gene Expression Patterns for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene Expression Patterns, 13(8), Phillips, J.B., Västinsalo, H., Wegner, J., Clément, A., Sankila, E.M., and Westerfield, M., The cone-dominant retina and the inner ear of zebrafish express the ortholog of CLRN1, the causative gene of human Usher syndrome type 3A, 473-81, Copyright (2013) with permission from Elsevier. Full text @ Gene Expr. Patterns