ZFIN ID: ZDB-FIG-131218-1
Phillips et al., 2013 - The cone-dominant retina and the inner ear of zebrafish express the ortholog of CLRN1, the causative gene of human Usher syndrome type 3A. Gene Expression Patterns   13(8):473-81 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene: clrn1
Fish: AB/TU
Anatomical Terms: anterior macula, anterior segment eye, auditory epithelial support cell, auditory receptor cell, brain (all 14) expand
Stage Range: Long-pec to Adult

Fig. 2

clrn1 is expressed in the retina, inner ear, and lateral line at embryonic, larval, and adult stages. A digoxigenin-labeled cRNA probe was used to detect clrn1 transcript on sectioned tissue from 2 dpf to 6 months of age. (A-C) Developing eyes and ears are enriched for clrn1 in the outer (closed arrow) and inner (open arrow) nuclear layers and ganglion cell layer (bracket) of the retina, (A and B), in mechanosensory hair cells (closed arrowhead in C) and supporting cells (open arrow in C) of the ear and in the neuromast cells (nm). (D-F) Retinal expression is retained through larval stages in all nuclear layers of the retina and in the proliferating cells of the ciliary marginal zone (open and closed arrows in D indicate inner nuclear layer and outer nuclear layer cells, respectively; bracket indicates the CMZ), all sensory patches of ear, and neuromasts (E). Additional expression is noted in brain (asterisk in F). 7 dpf larva in F was raised in PTU to suppress melanocyte formation. (G-I) 10 dpf zebrafish larvae express clrn1 in the anterior chamber of the retina (arrow in G) and continued expression is present in the photoreceptor layer (closed arrowhead in H) inner retina (open arrowhead in H) and CMZ (bracket in G). clrn1 expression is detected both in mechanosensory hair cells (closed arrowhead in I) and supporting cells (open arrow in I) of the sensory patches (anterior macula shown in I). (J and K) Continued expression of clrn1 transcript in the anterior chamber (open arrowhead in J), photoreceptors (closed arrow in K), and inner retinal cells (open arrow in K) of adult retinas. No signal is detected in tissues incubated with a sense probe (shown in L: 3 dpf larval retina treated with PTU). C,E,I: lateral views with anterior to the left; all others horizontal views, with anterior to the bottom. Abbreviations: am: anterior macula; lc: lateral crista; nm: neuromast. Scale bars: A-C, E, H, I and L: 20 µm. D, G, J and K: 50 µm. F: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
clrn1 AB/TU standard conditions Long-pec brain ISH
Long-pec eye ISH
Long-pec inner ear ISH
Protruding-mouth ciliary marginal zone ISH
Protruding-mouth lateral crista ISH
Protruding-mouth neuromast ISH
Protruding-mouth retinal ganglion cell layer ISH
Protruding-mouth retinal inner nuclear layer ISH
Protruding-mouth retinal outer nuclear layer ISH
Day 5 anterior macula ISH
Day 5 brain ISH
Day 5 ciliary marginal zone ISH
Day 5 neuromast ISH
Day 5 retinal ganglion cell layer ISH
Day 5 retinal inner nuclear layer ISH
Day 5 retinal outer nuclear layer ISH
Days 7-13 anterior macula ISH
Days 7-13 anterior segment eye ISH
Days 7-13 auditory epithelial support cell ISH
Days 7-13 auditory receptor cell ISH
Days 7-13 brain ISH
Days 7-13 ciliary marginal zone ISH
Days 7-13 neuromast ISH
Days 7-13 retinal ganglion cell layer ISH
Days 7-13 retinal inner nuclear layer ISH
Days 7-13 retinal outer nuclear layer ISH
Days 7-13 retinal photoreceptor layer ISH
Adult anterior segment eye ISH
Adult retinal inner nuclear layer ISH
Adult retinal outer nuclear layer ISH
Adult retinal photoreceptor layer ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Gene Expression Patterns for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene Expression Patterns, 13(8), Phillips, J.B., Västinsalo, H., Wegner, J., Clément, A., Sankila, E.M., and Westerfield, M., The cone-dominant retina and the inner ear of zebrafish express the ortholog of CLRN1, the causative gene of human Usher syndrome type 3A, 473-81, Copyright (2013) with permission from Elsevier. Full text @ Gene Expr. Patterns