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ZIRC
ZFIN ID: ZDB-FIG-131210-50
Zhen et al., 2013 - Hemogenic endothelium specification and hematopoietic stem cell maintenance employ distinct Scl isoforms. Development (Cambridge, England)   140(19):3977-3985 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Protruding-mouth

Fig. 6 scl-a7alpha; is required for the maintenance of HSCs in the AGM. (A-F) cmyb expression in control zebrafish embryos and scl-α morphants. Arrows indicate cmyb expression in AGM (A,B,D,E) or CHT (C,F) region. D, n=43/48; E, n=37/45; F, n=34/40. (G,H) CD41:eGFP+ HSCs in the AGM of 2 dpf control embryos and scl-7alpha; morphants (n=25/28). White arrows identify the CD41:eGFP+ HSCs and blue arrowheads indicate unaffected CD41:eGFP+ cells in the pronephric duct. (I-L2) Time-lapse confocal imaging of a live Tg(scl-α:DsRed; kdrl:eGFP) embryo injected with scl-α MO from <38 to 50 hpf. In J-L′, selected planes show three apoptotic HSCs in the scl-α morphants. Also see supplementary material Movie 4. Similar results were obtained in all four scl-α morphants observed (n=4/4). Scale bars: 50 μm in G,H; 20 μm in I-L2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed hkz07tTg; s843Tg + MO7-tal1 standard conditions Prim-25 to Long-pec hematopoietic stem cell IFL
Prim-25 to Long-pec nucleate erythrocyte IFL
EGFP hkz07tTg; s843Tg + MO7-tal1 standard conditions Prim-25 to Long-pec endothelial cell IFL
la2Tg control Long-pec pronephric duct cell IFL
Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
la2Tg + MO7-tal1 standard conditions Long-pec pronephric duct cell IFL
Long-pec Not Detected ventral wall of dorsal aorta hematopoietic stem cell IFL
myb WT control Prim-5 ventral wall of dorsal aorta hematopoietic stem cell ISH
Prim-25 ventral wall of dorsal aorta hematopoietic stem cell ISH
Protruding-mouth hematopoietic stem cell ISH
WT + MO7-tal1 standard conditions Prim-5 ventral wall of dorsal aorta hematopoietic stem cell ISH
Prim-25 ventral wall of dorsal aorta hematopoietic stem cell ISH
Protruding-mouth hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO7-tal1 standard conditions Prim-25 hematopoietic stem cell decreased amount, abnormal
Protruding-mouth hematopoietic stem cell decreased amount, abnormal
hkz07tTg; s843Tg + MO7-tal1 standard conditions Prim-25 - Long-pec hematopoietic stem cell apoptotic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development