ZFIN ID: ZDB-FIG-131031-23
Özhan et al., 2013 - Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains. Developmental Cell   26(4):331-345 Full text @ Dev. Cell
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 2

lypd6 Enhances Wnt/β-Catenin Signaling

(A) Classification of phenotypes caused by wnt8 overexpression in zebrafish embryos: class 1, small eyes (arrow); class 2, no eyes (arrow) but normal midbrain-hindbrain boundary (mhb) (arrowhead); class 3, severely reduced forebrain and midbrain and mhb not detectable (arrowhead); and class 4, loss of notochord.

(B) Phenotypes in hs:Wnt8 transgenic embryos injected with spGFP-lypd6 RNA (150 pg), spGFP-lypd6ΔGPI (140 pg), or equimolar amounts of spGFP-GPI control RNA and heat shocked at shield stage and scored at 24 hpf.

(C) pBAR luciferase reporter activity normalized to renilla luciferase levels in Wnt3a CM-treated HEK293T cells transfected with spGFP-Lypd6 (100 ng), spGFP-Lypd6ΔGPI (95 ng), or equimolar amounts of spGFP-GPI control. Error bars, SEM.

(D) Morphological phenotypes at 24 hpf of embryos injected with lypd6 MO (10 ng, 42/51 embryos) are rescued by coinjected lypd6 RNA (150 pg, 49/58 embryos).

(E) Rescue of phenotypes in hs:Wnt8 embryos injected with lypd6 MO (10 ng). Coinjected lypd6 RNA (150 pg) restores the more severe phenotypes.

(F) Reduction of reporter activity in 7xTcf:mCherry embryos injected with 10 ng lypd6 MO plus 100 ng control RNA (n = 28/31). Coinjected lypd6 RNA (75 ng) restores expression (n = 21/25).

(G) Downregulation of cdx4 (90% epiboly, 19/20) and sp5l (80% epiboly, 13/15) in embryos injected with 10 ng lypd6 MO.

See also Figure S2.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-lypd6 standard conditions Prim-5 caudal fin decreased size, abnormal
Prim-5 trunk decreased size, abnormal
w34Tg heat shock Prim-5 eye aplastic, abnormal
w34Tg + MO1-lypd6 heat shock Prim-5 eye aplastic, abnormal
Prim-5 eye decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 26(4), Özhan, G., Sezgin, E., Wehner, D., Pfister, A.S., Kühl, S.J., Kagermeier-Schenk, B., Kühl, M., Schwille, P., and Weidinger, G., Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains, 331-345, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell