ZFIN ID: ZDB-FIG-131031-24
Özhan et al., 2013 - Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains. Developmental Cell   26(4):331-345 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 50%-epiboly to Bud

Fig. 3

lypd6 Is Necessary for wnt8-Mediated Mesoderm and Neuroectoderm Patterning

(A) Expansion of the dorsal organizer domain (arrows) marked by goosecoid (gsc), not at late blastula (30% epiboly), but at early gastrula (60% epiboly) stage in embryos injected with 10 ng lypd6 MO.

(B) Quantification of the experiment shown in (A). Error bars, SEM.

(C) Changes in the size of the gsc expression domain (arrows) at 60% epiboly in embryos injected with low doses of wnt8.1 and wnt8.2 MOs (1.25 ng each) and lypd6 MO (2 ng), relative to embryos injected with equimolar amounts of control MO.

(D) gsc expression in embryos injected with high doses of wnt8 MOs (5 ng each) and 150 pg lypd6 RNA or equimolar amounts of GFP control RNA.

(E) Quantification of the experiments shown in (C) and (D). Error bars, SEM.

(F) Expansion of the forebrain marker otx2 and reduction of the posterior neural marker hoxb1b at the 100% epiboly stage in embryos injected with 10 ng lypd6 MO (19/23).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gsc WT standard conditions 30%-epiboly presumptive dorsal mesoderm ISH
50%-epiboly to 75%-epiboly presumptive dorsal mesoderm ISH
50%-epiboly to 75%-epiboly shield ISH
WT + MO1-lypd6 standard conditions 30%-epiboly presumptive dorsal mesoderm ISH
50%-epiboly to 75%-epiboly presumptive dorsal mesoderm ISH
50%-epiboly to 75%-epiboly shield ISH
WT + MO1-lypd6 + MO1-wnt8a + MO2-wnt8a standard conditions 50%-epiboly to 75%-epiboly presumptive dorsal mesoderm ISH
50%-epiboly to 75%-epiboly shield ISH
WT + MO1-wnt8a + MO2-wnt8a standard conditions 50%-epiboly to 75%-epiboly presumptive dorsal mesoderm ISH
50%-epiboly to 75%-epiboly shield ISH
hoxb1b WT standard conditions Bud neural plate posterior region ISH
WT + MO1-lypd6 standard conditions Bud neural plate posterior region ISH
otx2b WT standard conditions Bud forebrain neural plate ISH
WT + MO1-lypd6 standard conditions Bud forebrain neural plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 26(4), Özhan, G., Sezgin, E., Wehner, D., Pfister, A.S., Kühl, S.J., Kagermeier-Schenk, B., Kühl, M., Schwille, P., and Weidinger, G., Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains, 331-345, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell