ZFIN Figure: Zhang et al., 2013, Fig. 7

ZFIN ID: ZDB-FIG-131015-36

ZFIN ID: ZDB-FIG-131015-36

Zhang et al., 2013 - Tissue factor pathway inhibitor-2: A novel gene involved in zebrafish central nervous system development. Developmental Biology 381(1):38-49 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	EGFP elavl3 neurog1
Fish:	AB AB + MO1-tfpi2 knu3Tg knu3Tg + MO1-tfpi2
Knockdown Reagent:	MO1-tfpi2
Anatomical Terms:	neural plate neuron neuron whole organism
Stage Range:	1-4 somites to Prim-5

Fig. 7 ztfpi-2 morphants exhibited upregulation of huC and ngn1. (A) qPCR analysis showed huC and ngn1 levels have been increased in embryos injected with SP-MO at 25 hpf. ^*P<0.05. (B) WISH showed, at the 3-somite stage, injected embryos had a higher density of neurons in the neural plate than controls. (C) There were more HuC-positive cells in the SP-MO injected HuC-GFP embryos. A z-stack of fluorescence images was acquired by confocal microscopy at 25 hpf. Scale bar: 100 μm. (D) Dorsal views of Mcon-MO and SP-MO injected embryos showing ngn1 expression at 3 somites stage. The changes could be restored by co-injection of SP-MO with ztfpi-2 mRNA.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
EGFP	knu3Tg	control	Prim-5	neuron	IFL
	knu3Tg + MO1-tfpi2	standard conditions	Prim-5	neuron	IFL
elavl3	AB	control	1-4 somites	neural plate neuron	ISH
			Prim-5	whole organism	RTPCR
	AB + MO1-tfpi2	standard conditions	1-4 somites	neural plate neuron	ISH
			Prim-5	whole organism	RTPCR
neurog1	AB	control	1-4 somites	neuron	ISH
			Prim-5	whole organism	RTPCR
	AB + MO1-tfpi2	standard conditions	1-4 somites	neuron	ISH
			Prim-5	whole organism	RTPCR

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 381(1), Zhang, Y., Wang, L., Zhou, W., Wang, H., Zhang, J., Deng, S., Li, W., Li, H., Mao, Z., and Ma, D., Tissue factor pathway inhibitor-2: A novel gene involved in zebrafish central nervous system development, 38-49, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 381(1), Zhang, Y., Wang, L., Zhou, W., Wang, H., Zhang, J., Deng, S., Li, W., Li, H., Mao, Z., and Ma, D., Tissue factor pathway inhibitor-2: A novel gene involved in zebrafish central nervous system development, 38-49, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.