|ZFIN ID: ZDB-FIG-131015-36|
|Stage Range:||1-4 somites to Prim-5|
Fig. 7 ztfpi-2 morphants exhibited upregulation of huC and ngn1. (A) qPCR analysis showed huC and ngn1 levels have been increased in embryos injected with SP-MO at 25 hpf. *P<0.05. (B) WISH showed, at the 3-somite stage, injected embryos had a higher density of neurons in the neural plate than controls. (C) There were more HuC-positive cells in the SP-MO injected HuC-GFP embryos. A z-stack of fluorescence images was acquired by confocal microscopy at 25 hpf. Scale bar: 100 μm. (D) Dorsal views of Mcon-MO and SP-MO injected embryos showing ngn1 expression at 3 somites stage. The changes could be restored by co-injection of SP-MO with ztfpi-2 mRNA.
|knu3Tg + MO1-tfpi2||standard conditions||Prim-5||neuron||IFL|
|elavl3||AB||control||1-4 somites||neural plate neuron||ISH|
|AB + MO1-tfpi2||standard conditions||1-4 somites||neural plate neuron||ISH|
|AB + MO1-tfpi2||standard conditions||1-4 somites||neuron||ISH|
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Reprinted from Developmental Biology, 381(1), Zhang, Y., Wang, L., Zhou, W., Wang, H., Zhang, J., Deng, S., Li, W., Li, H., Mao, Z., and Ma, D., Tissue factor pathway inhibitor-2: A novel gene involved in zebrafish central nervous system development, 38-49, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.