ZFIN ID: ZDB-FIG-131015-36
Zhang et al., 2013 - Tissue factor pathway inhibitor-2: A novel gene involved in zebrafish central nervous system development. Developmental Biology   381(1):38-49 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 1-4 somites to Prim-5

Fig. 7 ztfpi-2 morphants exhibited upregulation of huC and ngn1. (A) qPCR analysis showed huC and ngn1 levels have been increased in embryos injected with SP-MO at 25 hpf. *P<0.05. (B) WISH showed, at the 3-somite stage, injected embryos had a higher density of neurons in the neural plate than controls. (C) There were more HuC-positive cells in the SP-MO injected HuC-GFP embryos. A z-stack of fluorescence images was acquired by confocal microscopy at 25 hpf. Scale bar: 100 μm. (D) Dorsal views of Mcon-MO and SP-MO injected embryos showing ngn1 expression at 3 somites stage. The changes could be restored by co-injection of SP-MO with ztfpi-2 mRNA.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg control Prim-5 neuron IFL
knu3Tg + MO1-tfpi2 standard conditions Prim-5 neuron IFL
elavl3 AB control 1-4 somites neural plate neuron ISH
Prim-5 whole organism RTPCR
AB + MO1-tfpi2 standard conditions 1-4 somites neural plate neuron ISH
Prim-5 whole organism RTPCR
neurog1 AB control 1-4 somites neuron ISH
Prim-5 whole organism RTPCR
AB + MO1-tfpi2 standard conditions 1-4 somites neuron ISH
Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 381(1), Zhang, Y., Wang, L., Zhou, W., Wang, H., Zhang, J., Deng, S., Li, W., Li, H., Mao, Z., and Ma, D., Tissue factor pathway inhibitor-2: A novel gene involved in zebrafish central nervous system development, 38-49, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.