|ZFIN ID: ZDB-FIG-130802-8|
|Stage Range:||26+ somites to High-pec|
Quantitative RT-PCR analysis of dusp6 activation levels in Tg(hsp70-HRASG12V) at various time points after 1 hour of heat shock. Transgenic embryos were kept at 28.5°C until 22 hpf. Some received 37°C heat shock for 1 hour; some were kept as controls. At 4, 6, 8 and 24 hours post-heat shock, the embryos were harvested for RNA extraction and RT-PCR analysis. Transgenic embryos kept at 28.5°C without heat shock were used as controls.
|dusp6||pd8Tg||control||26+ somites to High-pec||whole organism||RTPCR|
|pd8Tg||heat shock||26+ somites to High-pec||whole organism||RTPCR|
|Acknowledgments:||ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development|