ZFIN ID: ZDB-FIG-130802-8
Le et al., 2013 - A novel chemical screening strategy in zebrafish identifies common pathways in embryogenesis and rhabdomyosarcoma development. Development (Cambridge, England)   140(11):2354-64 Full text @ Development
Anatomical Term:
Stage Range: 26+ somites to High-pec

Fig. S4

Quantitative RT-PCR analysis of dusp6 activation levels in Tg(hsp70-HRASG12V) at various time points after 1 hour of heat shock. Transgenic embryos were kept at 28.5°C until 22 hpf. Some received 37°C heat shock for 1 hour; some were kept as controls. At 4, 6, 8 and 24 hours post-heat shock, the embryos were harvested for RNA extraction and RT-PCR analysis. Transgenic embryos kept at 28.5°C without heat shock were used as controls.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dusp6 pd8Tg control 26+ somites to High-pec whole organism RTPCR
pd8Tg heat shock 26+ somites to High-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development