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ZFIN ID: ZDB-FIG-130725-27
Le et al., 2013 - A novel chemical screening strategy in zebrafish identifies common pathways in embryogenesis and rhabdomyosarcoma development. Development (Cambridge, England)   140(11):2354-64 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: 26+ somites

Fig. 1 Transient RAS action in Tg(hsp70-HRASG12V) zebrafish embryos resembles pathway activation during tumorigenesis. (A) Ingenuity pathway analysis output for the upregulated gene set identified on comparing Tg(hsp70-HRASG12V) embryos to wild-type zebrafish embryos following heat shock (log fold change >0.5 for top network and >0.7 for top diseases and disorders). (B) RAS signature gene list, defined as commonly upregulated genes in zebrafish RMS (white circle), human embryonal RMS (blue circle), human pancreatic adenocarcinoma (red circle) and RAS-infected HMECs (yellow circle). The total number of genes is shown, with the number tested in the 17-gene list in parentheses [adapted from Langenau et al. (Langenau et al., 2007)]. (C) RT-PCR analysis of six of the RAS signature genes in Tg(hsp70-HRASG12V) and wild-type embryos with and without heat shock. P<0.05 for each gene shown, HRAS+HS compared with other conditions. (D) ISH of erm. Transgenic embryos were incubated with various chemicals from 16 hpf, heat shocked at 18-19 hpf, and fixed at 24 hpf. DMSO, vehicle control. (E) RT-PCR analysis of erm and dusp6 expression levels in response to pathway-specific chemical inhibitors. Error bars indicate s.e.m. HS, heat shock; NoHS, no heat shock.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ddx18 WT control 26+ somites whole organism RTPCR
WT heat shock 26+ somites whole organism RTPCR
pd8Tg control 26+ somites whole organism RTPCR
pd8Tg heat shock 26+ somites whole organism RTPCR
dusp4 WT control 26+ somites whole organism RTPCR
WT heat shock 26+ somites whole organism RTPCR
pd8Tg control 26+ somites whole organism RTPCR
pd8Tg heat shock 26+ somites whole organism RTPCR
dusp6 WT control 26+ somites whole organism RTPCR
WT heat shock 26+ somites whole organism RTPCR
pd8Tg control 26+ somites whole organism RTPCR
pd8Tg heat shock 26+ somites whole organism RTPCR
fgfr4 WT control 26+ somites whole organism RTPCR
WT heat shock 26+ somites whole organism RTPCR
pd8Tg control 26+ somites whole organism RTPCR
pd8Tg heat shock 26+ somites whole organism RTPCR
mcl1a WT control 26+ somites whole organism RTPCR
WT heat shock 26+ somites whole organism RTPCR
pd8Tg control 26+ somites whole organism RTPCR
pd8Tg heat shock 26+ somites whole organism RTPCR
sat1a.2 WT control 26+ somites whole organism RTPCR
WT heat shock 26+ somites whole organism RTPCR
pd8Tg control 26+ somites whole organism RTPCR
pd8Tg heat shock 26+ somites whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development