ZFIN Figure: Wang et al., 2013, Fig. 7

ZFIN ID: ZDB-FIG-130624-21

ZFIN ID: ZDB-FIG-130624-21

Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish. Journal of Cell Science 126(Pt 6):1381-91 Full text @ J. Cell Sci.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	GFP mCherry
Fish:	jh11Tg; la116Tg fbn2b^{te382a/te382a}; pku300^{te382b/te382b}; jh11Tg; la116Tg
Anatomical Terms:	atrioventricular canal endocardium cardiac ventricle endocardium ventricular endocardium
Stage Range:	Long-pec to Protruding-mouth

PHENOTYPE:

Fish:	fbn2b^{te382a/te382a}; pku300^{te382b/te382b}; jh11Tg; la116Tg
Observed In:	atrioventricular canal Notch signaling involved in heart development
Stage Range:	Long-pec to Protruding-mouth

Fig. 7 Notch signaling was not activated in the AVC of sco^te382 mutant hearts. (A–F) Tg(Tp1bglob:hmgb1-mCherry)^jh11, called Tg(Tp1:mCherry), transgenic zebrafish (Parsons et al., 2009) were bred with sco^te382/te382; Tg(kdrl:eGFP) transgenic zebrafish to generate sco^te382/+; Tg(Tp1:mCherry)/+; Tg(kdrl:eGFP)/+ zebrafish. Images were taken with agarose-embedded embryos using Zeiss 700 upright confocal microscope. Endocardial cells labeled by Tg(kdrl:eGFP) were used to project the heart shapes. Note that Tg(Tp1:mCherry) were gradually restricted in the AVC of wild-type hearts at 48hpf (A), 60hpf (C) to 72hpf (E), but were ectopically expressed in the ventricle of sco^te382 mutant hearts at 48hpf (B), 60hpf (D) and 72hpf (F). a, atrium; v, ventricle. Red arrowheads point to the AVC.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
GFP	fbn2b^{te382a/te382a}; pku300^{te382b/te382b}; jh11Tg; la116Tg	standard conditions	Long-pec		endocardium	IFL
			Pec-fin		endocardium	IFL
			Protruding-mouth		endocardium	IFL
	jh11Tg; la116Tg	standard conditions	Long-pec		endocardium	IFL
			Pec-fin		endocardium	IFL
			Protruding-mouth		endocardium	IFL
mCherry	fbn2b^{te382a/te382a}; pku300^{te382b/te382b}; jh11Tg; la116Tg	standard conditions	Long-pec	Not Detected	atrioventricular canal endocardium	IFL
			Long-pec		cardiac ventricle	IFL
			Pec-fin	Not Detected	atrioventricular canal endocardium	IFL
			Pec-fin		cardiac ventricle	IFL
			Protruding-mouth	Not Detected	atrioventricular canal endocardium	IFL
			Protruding-mouth		cardiac ventricle	IFL
	jh11Tg; la116Tg	standard conditions	Long-pec		atrioventricular canal endocardium	IFL
			Long-pec		ventricular endocardium	IFL
			Pec-fin		atrioventricular canal endocardium	IFL
			Protruding-mouth		atrioventricular canal endocardium	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
fbn2b^{te382a/te382a}; pku300^{te382b/te382b}; jh11Tg; la116Tg	standard conditions	Long-pec	atrioventricular canal cellular quality, abnormal
		Long-pec	Notch signaling involved in heart development process quality, abnormal
		Pec-fin	atrioventricular canal cellular quality, abnormal
		Pec-fin	Notch signaling involved in heart development process quality, abnormal
		Protruding-mouth	atrioventricular canal cellular quality, abnormal
		Protruding-mouth	Notch signaling involved in heart development process quality, abnormal

Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.