ZFIN ID: ZDB-FIG-130624-21 |
Genes: | |
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Fish: | |
Anatomical Terms: | |
Stage Range: | Long-pec to Protruding-mouth |
Fish: | |
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Observed In: | |
Stage Range: | Long-pec to Protruding-mouth |
Fig. 7 Notch signaling was not activated in the AVC of scote382 mutant hearts. (A–F) Tg(Tp1bglob:hmgb1-mCherry)jh11, called Tg(Tp1:mCherry), transgenic zebrafish (Parsons et al., 2009) were bred with scote382/te382; Tg(kdrl:eGFP) transgenic zebrafish to generate scote382/+; Tg(Tp1:mCherry)/+; Tg(kdrl:eGFP)/+ zebrafish. Images were taken with agarose-embedded embryos using Zeiss 700 upright confocal microscope. Endocardial cells labeled by Tg(kdrl:eGFP) were used to project the heart shapes. Note that Tg(Tp1:mCherry) were gradually restricted in the AVC of wild-type hearts at 48hpf (A), 60hpf (C) to 72hpf (E), but were ectopically expressed in the ventricle of scote382 mutant hearts at 48hpf (B), 60hpf (D) and 72hpf (F). a, atrium; v, ventricle. Red arrowheads point to the AVC. |
Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
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GFP | fbn2bte382a/te382a; pku300te382b/te382b; jh11Tg; la116Tg | standard conditions | Long-pec | endocardium | IFL | ||
Pec-fin | endocardium | IFL | |||||
Protruding-mouth | endocardium | IFL | |||||
jh11Tg; la116Tg | standard conditions | Long-pec | endocardium | IFL | |||
Pec-fin | endocardium | IFL | |||||
Protruding-mouth | endocardium | IFL | |||||
mCherry | fbn2bte382a/te382a; pku300te382b/te382b; jh11Tg; la116Tg | standard conditions | Long-pec | Not Detected | atrioventricular canal endocardium | IFL | |
Long-pec | cardiac ventricle | IFL | |||||
Pec-fin | Not Detected | atrioventricular canal endocardium | IFL | ||||
Pec-fin | cardiac ventricle | IFL | |||||
Protruding-mouth | Not Detected | atrioventricular canal endocardium | IFL | ||||
Protruding-mouth | cardiac ventricle | IFL | |||||
jh11Tg; la116Tg | standard conditions | Long-pec | atrioventricular canal endocardium | IFL | |||
Long-pec | ventricular endocardium | IFL | |||||
Pec-fin | atrioventricular canal endocardium | IFL | |||||
Protruding-mouth | atrioventricular canal endocardium | IFL |
Acknowledgments: | ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci. |
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