ZFIN ID: ZDB-FIG-130624-21
Wang et al., 2013 - Genetic Interaction between pku300 and fbn2b Controls Endocardial Cell Proliferation and Valve Development in Zebrafish. Journal of Cell Science   126(Pt 6):1381-91 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 7 Notch signaling was not activated in the AVC of scote382 mutant hearts. (AF) Tg(Tp1bglob:hmgb1-mCherry)jh11, called Tg(Tp1:mCherry), transgenic zebrafish (Parsons et al., 2009) were bred with scote382/te382; Tg(kdrl:eGFP) transgenic zebrafish to generate scote382/+; Tg(Tp1:mCherry)/+; Tg(kdrl:eGFP)/+ zebrafish. Images were taken with agarose-embedded embryos using Zeiss 700 upright confocal microscope. Endocardial cells labeled by Tg(kdrl:eGFP) were used to project the heart shapes. Note that Tg(Tp1:mCherry) were gradually restricted in the AVC of wild-type hearts at 48hpf (A), 60hpf (C) to 72hpf (E), but were ectopically expressed in the ventricle of scote382 mutant hearts at 48hpf (B), 60hpf (D) and 72hpf (F). a, atrium; v, ventricle. Red arrowheads point to the AVC.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP fbn2bte382a/te382a; pku300te382b/te382b; jh11Tg; la116Tg standard conditions Long-pec endocardium IFL
Pec-fin endocardium IFL
Protruding-mouth endocardium IFL
jh11Tg; la116Tg standard conditions Long-pec endocardium IFL
Pec-fin endocardium IFL
Protruding-mouth endocardium IFL
mCherry fbn2bte382a/te382a; pku300te382b/te382b; jh11Tg; la116Tg standard conditions Long-pec Not Detected atrioventricular canal endocardium IFL
Long-pec cardiac ventricle IFL
Pec-fin Not Detected atrioventricular canal endocardium IFL
Pec-fin cardiac ventricle IFL
Protruding-mouth Not Detected atrioventricular canal endocardium IFL
Protruding-mouth cardiac ventricle IFL
jh11Tg; la116Tg standard conditions Long-pec atrioventricular canal endocardium IFL
Long-pec ventricular endocardium IFL
Pec-fin atrioventricular canal endocardium IFL
Protruding-mouth atrioventricular canal endocardium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fbn2bte382a/te382a; pku300te382b/te382b; jh11Tg; la116Tg standard conditions Long-pec atrioventricular canal cellular quality, abnormal
Long-pec Notch signaling involved in heart development process quality, abnormal
Pec-fin atrioventricular canal cellular quality, abnormal
Pec-fin Notch signaling involved in heart development process quality, abnormal
Protruding-mouth atrioventricular canal cellular quality, abnormal
Protruding-mouth Notch signaling involved in heart development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.