ZFIN Figure: Villefranc et al., 2013, Fig. 2

ZFIN ID: ZDB-FIG-130618-17

ZFIN ID: ZDB-FIG-130618-17

Villefranc et al., 2013 - A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development. Development (Cambridge, England) 140(7):1497-1506 Full text @ Development

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	EGFP
Fish:	y1Tg kdrl^y17/+; y1Tg/y1Tg vegfc^um18/um18; y1Tg/y1Tg kdrl^y17/y17; vegfc^um18/um18; y1Tg
Anatomical Terms:	dorsal aorta intersegmental vessel posterior cardinal vein
Stage:	Prim-15

PHENOTYPE:

Fish:	kdrl^y17/+; y1Tg/y1Tg vegfc^um18/um18; y1Tg/y1Tg kdrl^y17/+; vegfc^um18/+; y1Tg/y1Tg kdrl^y17/y17; vegfc^um18/um18; y1Tg
Observed In:	angiogenesis intersegmental vessel
Stage:	Prim-15

Fig. 2 vegfc^um18 enhances angiogenesis defects in kdrl^y17 mutant embryos. (A-D) Confocal micrographs of trunk blood vessels in Tg(fli1a:egfp)^y1 (A) wild-type, (B) vegfc^um18 mutant, (C) kdr^y17 mutant and (D) kdr^y17 vegfc^um18 double-mutant zebrafish embryos at 30 hpf. Arrows indicate intersomitic vessels (ISVs). Anterior is to the left, dorsal is up. (E) ISV length in embryos of the indicated genotype at 30 hpf. Values are the average from two clutches of embryos. ^*P<0.05; N.S., not significant; error bars indicate s.e.m. DA, dorsal aorta; PCV, posterior cardinal vein. Scale bar: 50 μm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
EGFP	kdrl^y17/+; y1Tg/y1Tg	standard conditions	Prim-15	dorsal aorta	IFL
			Prim-15	intersegmental vessel	IFL
			Prim-15	posterior cardinal vein	IFL
	kdrl^y17/y17; vegfc^um18/um18; y1Tg	standard conditions	Prim-15	dorsal aorta	IFL
			Prim-15	posterior cardinal vein	IFL
	vegfc^um18/um18; y1Tg/y1Tg	standard conditions	Prim-15	dorsal aorta	IFL
			Prim-15	intersegmental vessel	IFL
			Prim-15	posterior cardinal vein	IFL
	y1Tg	standard conditions	Prim-15	dorsal aorta	IFL
			Prim-15	intersegmental vessel	IFL
			Prim-15	posterior cardinal vein	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
kdrl^y17/+; vegfc^um18/+; y1Tg/y1Tg	standard conditions	Prim-15	angiogenesis disrupted, abnormal
		Prim-15	intersegmental vessel decreased length, abnormal
kdrl^y17/+; y1Tg/y1Tg	standard conditions	Prim-15	angiogenesis disrupted, abnormal
		Prim-15	intersegmental vessel decreased length, abnormal
kdrl^y17/y17; vegfc^um18/um18; y1Tg	standard conditions	Prim-15	angiogenesis disrupted, abnormal
		Prim-15	intersegmental vessel decreased length, abnormal
vegfc^um18/um18; y1Tg/y1Tg	standard conditions	Prim-15	intersegmental vessel length, normal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development