ZFIN ID: ZDB-FIG-130510-10
Münch et al., 2013 - Notch regulates blastema proliferation and prevents differentiation during adult zebrafish fin regeneration. Development (Cambridge, England)   140(7):1402-1411 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage: Adult

Fig. 3

Notch signalling inactivation impairs regeneration by decreasing proliferation. (A,B) Fish were treated with DMSO or 10 µM RO4929097 for 3 days starting at 1 hpa. Fin regeneration is blocked by RO4929097 treatment. (C) Mean length of fin regenerates (n=5); **P<0.01. (D,E) In situ hybridization of her6 showing Notch activity in regenerating fins treated with DMSO from 62 to 72 hpa (D) but not in RO4929097-treated fins (E). (F-H) BrdU-stained fin sections and quantification of BrdU+ cells within the mesenchyme of RO4929097-treated fins (G; n=5) and DMSO-treated fins (F; n=6). *P<0.05. (I-M) Fins microinjected in the dorsal half with fluorescein-labelled morpholinos (MO, green) and electroporated at 2 days post-transfection (dpt): control (I; n=9), jag1b (J; n=12), notch1b (K; n=9), lfng (L; n=12) or rbpjκ (M; n=6). The ventral half serves as an internal control. Pink brackets mark maximal regenerative outgrowth at 2 dpt. (N) Mean outgrowth size of the dorsal (MO-electroporated) half of the fin relative to the ventral half at 4 dpa/2 dpt. *P<0.05, **P<0.01 and ***P<0.005 versus control. (O,P) BrdU-stained fin sections and quantification of BrdU+ cells within the mesenchyme. (Q) Mean percentage of DAPI+ blastema cells in fin sections incorporating BrdU at 3 dpa/24 hpt (n=3). *P<0.05. Proliferation is reduced in notch1b-MO-transfected fin halves. Scale bars: 1 mm in B; 10 μm in E,G; 1 mm in M; 10 μm in P. Broken lines indicate the amputation plane.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
her6 WT physical alteration: anatomical structure Adult blastema ISH
Adult regenerating fin ISH
WT physical alteration: anatomical structure, chemical treatment: pharmaceutical Adult Not Detected blastema ISH
Adult Not Detected regenerating fin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT physical alteration: anatomical structure, chemical treatment: pharmaceutical Adult blastema cell population proliferation decreased process quality, abnormal
Adult fin regeneration decreased process quality, abnormal
WT + MO1-jag1b physical alteration: anatomical structure Adult fin regeneration decreased process quality, abnormal
WT + MO1-notch1b physical alteration: anatomical structure Adult blastema cell population proliferation decreased process quality, abnormal
Adult fin regeneration decreased process quality, abnormal
WT + MO2-lfng physical alteration: anatomical structure Adult fin regeneration decreased process quality, abnormal
WT + MO4-rbpja,rbpjb physical alteration: anatomical structure Adult fin regeneration decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development