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ZFIN ID: ZDB-FIG-130429-6
Liu et al., 2013 - Eaf1 and Eaf2 negatively regulate canonical Wnt/β-catenin signaling. Development (Cambridge, England)   140(5):1067-1078 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Term:
Stage: Bud
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage: Bud

Fig. 2 Eafs antagonize Wnt/β-catenin signaling in anterior neuroectoderm patterning. (A) eaf1 and eaf2 counteract wnt8a-mediated forebrain defects. (a) In situ hybridization for the anterior neuroectoderm marker opl in wild type (WT) and in the presence of wnt8a mRNA alone or together with eaf1 or eaf2 mRNA. (b) Scoring of opl expression: black, reduced; white, normal; gray, expanded. (B) eaf1 or eaf2 partially counteracts the strong reduction in opl expression that occurs after exposure to the Gsk3 inhibitor BIO, which enhances Wnt/β-catenin signaling. opl expression is scored as in A. (C) eaf1 and eaf2 rescue forebrain defects in apc mutants. opl expression is scored as: black, strongly reduced; blue, reduced; white, normal; gray, increased. (D) Heat shock-induced expression of dkk1 at 50% epiboly rescues forebrain defects in Eaf morphants. opl expression is scored as in A. (E) dn-Tcf partially rescues the anterior brain defects in Eaf morphants. Embryos were scored by six3b expression: black, reduced; white, normal; gray, increased. Aa,Ba,Ea, dorsal views, anterior to the left; Ca,Da, lateral views, anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
zic1 WT standard conditions Bud neuroectoderm anterior region ISH
WT chemical treatment: drug Bud Not Detected neuroectoderm anterior region ISH
WT + MO1-eaf1 standard conditions Bud neuroectoderm anterior region ISH
WT + MO1-eaf2 standard conditions Bud neuroectoderm anterior region ISH
apchu745/hu745 standard conditions Bud neuroectoderm anterior region ISH
w32Tg control Bud neuroectoderm anterior region ISH
w32Tg heat shock Bud neuroectoderm anterior region ISH
w32Tg + MO1-eaf1 heat shock Bud neuroectoderm anterior region ISH
w32Tg + MO1-eaf2 heat shock Bud neuroectoderm anterior region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: drug Bud neuroectoderm anterior region absent, abnormal
WT + MO1-eaf1 standard conditions Bud neuroectoderm anterior region decreased size, abnormal
WT + MO1-eaf2 standard conditions Bud neuroectoderm anterior region decreased size, abnormal
apchu745/hu745 standard conditions Bud neuroectoderm anterior region decreased size, abnormal
w32Tg control Bud neuroectoderm anterior region size, normal
w32Tg heat shock Bud neuroectoderm anterior region increased size, abnormal
w32Tg + MO1-eaf1 heat shock Bud neuroectoderm anterior region increased size, abnormal
w32Tg + MO1-eaf2 heat shock Bud neuroectoderm anterior region increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development