ZFIN ID: ZDB-FIG-130423-9
Stoll et al., 2013 - HOXC9 Regulates Formation of Parachordal Lymphangioplasts and the Thoracic Duct in Zebrafish via Stabilin 2. PLoS One   8(3):e58311 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 5 HOXC9 overexpression rescues the defects in parachordal lymphangioplast (PL) formation in Stab1 morphants.
(A) Overall morphology of 48 hpf zebrafish embryo after control morpholino injection. Red box shows region displayed in (B–D). (B) Normal formation of the PLs (arrows) in 48 hpf tg(fli1:EGFP) fish embryo after injection of 4 ng control morpholino. (C) Silencing of Stab1 expression using 4 ng splice-blocking morpholino disrupted the formation of the PLs (asterisks) in 48 hpf tg(fli1:EGFP) zebrafish embryo. (D) Injection of HOXC9 mRNA (50 pg) rescued the Stab1 loss-of-function phenotype in 48 hpf tg(fli1:EGFP) zebrafish embryo. (E) Quantification of 48 hpf tg(fli1:EGFP) zebrafish embryos showing a disturbed PL formation including rescue experiments using HOXC9 mRNA (50 pg). Embryos were divided in three groups depending on the PL appearance being completely absent, partially formed or completely present. (F) RT-PCR analysis for increased expression of Stab2 in zebrafish injected with the Stab1-Ex3-Mo (4 ng) and HOXC9 mRNA (50 pg). (G) Quantification of (F), n = 3 per group. *p<0.05 vs. Co-Mo. Black scale bar: 500 μm. White scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Long-pec common cardinal vein IFL
Long-pec dorsal aorta IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec vascular lymphangioblast IFL
y1Tg + MO2-stab1 standard conditions Long-pec common cardinal vein IFL
Long-pec dorsal aorta IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec vascular lymphangioblast IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO2-stab1 standard conditions Long-pec lymphangiogenesis arrested, abnormal
Long-pec vascular lymphangioblast hypoplastic, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One