ZFIN ID: ZDB-FIG-130423-10
Stoll et al., 2013 - HOXC9 Regulates Formation of Parachordal Lymphangioplasts and the Thoracic Duct in Zebrafish via Stabilin 2. PLoS One   8(3):e58311 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 6 Silencing of HOXC9, Stab1 and Stab2 expression in zebrafish inhibits formation of the thoracic duct (TD).
(A) 120 hpf tg(fli1:EGFP) zebrafish embryo. Blue box marks the region magnified in (A2). (A2) Trunk vasculature of the embryo shown in (A). Red box marks the region magnified in (B–J). (B) Normal formation of the TD (arrows and dotted line) in 120 hpf tg(fli1:EGFP) zebrafish embryos after injection of control morpholino. Blue bar marks dorsal aorta and red bar marks cardinal vein. (C–G) Silencing of HOXC9, Stab1 and Stab2 expression using the indicated morpholinos disrupted the formation of the TD (asterisks) in 120 hpf tg(fli1:EGFP) zebrafish embryos. (H–J) Co-injection of 50 pg HOXC9 mRNA rescued the defects in TD formation caused by silencing of HOXC9, Stab1 and Stab2 using the indicated morpholinos. (K) Quantification of defects in TD formation of embryos shown in (A–J) including rescue experiments with 50 pg HOXC9-mRNA. Black scale bar: 500 μm. White scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Day 5 common cardinal vein IFL
Day 5 dorsal aorta IFL
Day 5 thoracic duct IFL
Day 5 trunk vasculature IFL
y1Tg standard conditions Day 5 common cardinal vein IFL
Day 5 dorsal aorta IFL
Day 5 thoracic duct IFL
Day 5 trunk vasculature IFL
y1Tg + MO1-hoxc9a standard conditions Day 5 common cardinal vein IFL
Day 5 dorsal aorta IFL
Day 5 thoracic duct IFL
Day 5 trunk vasculature IFL
y1Tg + MO1-stab1 standard conditions Day 5 common cardinal vein IFL
Day 5 dorsal aorta IFL
Day 5 thoracic duct IFL
Day 5 trunk vasculature IFL
y1Tg + MO2-stab1 standard conditions Day 5 common cardinal vein IFL
Day 5 dorsal aorta IFL
Day 5 thoracic duct IFL
Day 5 trunk vasculature IFL
y1Tg + MO2-stab2 standard conditions Day 5 common cardinal vein IFL
Day 5 dorsal aorta IFL
Day 5 thoracic duct IFL
Day 5 trunk vasculature IFL
y1Tg + MO3-stab2 standard conditions Day 5 common cardinal vein IFL
Day 5 dorsal aorta IFL
Day 5 thoracic duct IFL
Day 5 trunk vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-hoxc9a standard conditions Day 5 lymphangiogenesis arrested, abnormal
Day 5 thoracic duct hypoplastic, abnormal
y1Tg + MO1-stab1 standard conditions Day 5 lymphangiogenesis arrested, abnormal
Day 5 thoracic duct hypoplastic, abnormal
y1Tg + MO2-stab1 standard conditions Day 5 lymphangiogenesis arrested, abnormal
Day 5 thoracic duct hypoplastic, abnormal
y1Tg + MO2-stab2 standard conditions Day 5 lymphangiogenesis arrested, abnormal
Day 5 thoracic duct hypoplastic, abnormal
y1Tg + MO3-stab2 standard conditions Day 5 lymphangiogenesis arrested, abnormal
Day 5 thoracic duct hypoplastic, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One