ZFIN ID: ZDB-FIG-130321-10
Cheesman et al., 2011 - Epithelial cell proliferation in the developing zebrafish intestine is regulated by the Wnt pathway and microbial signaling via Myd88. Proceedings of the National Academy of Sciences of the United States of America   108(Suppl 1):4570-4577 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagent:
Observed In:
Stage: Day 6

Fig. 4

Combinatorial effects of microbial and Wnt signaling on intestinal epithelial cell proliferation. (A-F) S-phase intestinal epithelial cells were quantified in 30 serial sections in the intestinal bulb of individual larvae as in Fig. 1. To label S-phase nuclei, the experiments in A and D used BrdU and the experiments in B, C, E, and F used EdU. All experiments were performed with 6-dpf larvae except the experiment in D, which was performed with 8-dpf larvae. (A) CV axin1 had significantly more proliferating intestinal cells than CV WT (wt), GF wt, and GF axin1 (P < 0.0001). GF axin1 had significantly more proliferating cells than GF wt (P < 0.01). (B) axin1 had significantly more proliferating cells than wt, myd88 MO, and axin1 myd88 MO (P < 0.001). axin1 myd88 MO had significantly more proliferating cells than myd88 MO (P < 0.001). (C) Exposure of GF larvae from 3 to 6 dpf to 30 µg/mL LPS caused no change in cell proliferation relative to untreated GF larvae. (D) Monoassociation of GF larvae with A. veronii (AMA) or treatment with 500 ng/mL A. veronii CFS induced a significant increase in intestinal cell proliferation relative to GF (P < 0.0001). (E) axin1 mutants monoassociated with A. veronii had significantly more proliferating cells than CV axin1 (P < 0.0001). (F) tcf4 mutants had significantly fewer dividing intestinal cells than wt siblings when reared GF (P < 0.01) and when monoassociated with A. veronii (P < 0.0001). Intestinal epithelial cells with cytoplasmic β-catenin were quantified in 30 serial sections in the intestinal bulb of 6-dpf larvae. (G and H) CV wt larvae had significantly more β-catenin-positive cells than GF wt larvae (P < 0.05). (G) CV axin1 had significantly more β-catenin-positive cells than GF axin1 (P < 0.05). (H) A. veronii monoassociated larvae had significantly more β-catenin-positive cells than CV animals (P < 0.0001).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
axin1tm213/tm213 control Day 6 epithelial cell proliferation increased occurrence, abnormal
Day 6 intestinal bulb epithelium cellular quality, abnormal
axin1tm213/tm213 bacterial treatment: Aeromonas veronii Day 6 epithelial cell proliferation increased occurrence, abnormal
Day 6 intestinal bulb epithelium cellular quality, abnormal
axin1tm213/tm213 germ free Day 6 epithelial cell proliferation increased occurrence, abnormal
Day 6 intestinal bulb epithelium cellular quality, abnormal
axin1tm213/tm213 + MO2-myd88 standard conditions Day 6 epithelial cell proliferation increased occurrence, abnormal
Day 6 intestinal bulb epithelium cellular quality, abnormal
tcf7l2zf55/zf55 bacterial treatment: Aeromonas veronii Day 6 epithelial cell proliferation decreased occurrence, abnormal
Day 6 intestinal bulb epithelium cellular quality, abnormal
tcf7l2zf55/zf55 germ free Day 6 epithelial cell proliferation decreased occurrence, abnormal
Day 6 intestinal bulb epithelium cellular quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA