FIGURE

Fig. S1

ID

ZDB-FIG-101129-10

Publication

Cheesman et al., 2011 - Epithelial cell proliferation in the developing zebrafish intestine is regulated by the Wnt pathway and microbial signaling via Myd88

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Fig. S1

Wnt signaling occurs in the larval zebrafish intestine. Amplification of Wnt target genes and reporter from 6 dpf WT (A) and topD (B) larvae. The relative cycle threshold (Ct) values for a housekeeping gene (rp32) and the indicated Wnt target genes axin1, cdx1a, and nt1 (A) or the gfp reporter (B) are shown. The mean and SD of three triplicate reactions are shown. For all primer pairs, a no-template control reaction yielded no detectable product (i.e., Ct = 40). (C) Relative abundance (log2) of c-myc amplified from axin1 vs. WT (wt) 6-dpf larval intestines is shown. Levels of c-myc were normalized to levels of rp32 for each sample. Error bars represent upper and lower limits, based on the SD of the ΔCt values. (D and E) Intestines of live 6-dpf wt and axin1 larvae. The axin1 intestine appeared thicker and more convoluted.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	axin1^tm213/tm213
Observed In:	intestine
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA