FIGURE

Fig. 4

ID

ZDB-FIG-130115-23

Publication

Watanabe et al., 2012 - In vivo assessment of the permeability of the blood--brain barrier and blood-retinal barrier to fluorescent indoline derivatives in zebrafish

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Fig. 4

Effect of MK571 on the permeability of the BBB and BRB to ZMJ018. Zebrafish larvae (albino line) at 7-8 dpf were immersed in egg water containing 1 μM of ZMJ018 with and without 30 μM of MK571 for 4 h. A-C: Schematic diagram showing the regions observed using a CLSM. D-I: In vivo fluorescence imaging of the OT (D and G), HBV (E and H) and multiple layers of the retina (F and I) in zebrafish stained with ZMJ018 only (D-F) or ZMJ018 in the presence of MK571 (G-I). The OT and multiple layers of the retina were clearly visible in zebrafish stained with ZMJ018 in the presence of MK571. J-L: Quantitative analysis of the FI in the OT (J) and HBV (K), and the ratio of the FI (L). Both the FI in the OT and the relative FI (OT/HBV) were significantly higher in zebrafish stained with ZMJ018 in the presence of MK571 (n = 4, *P < 0.05). Scale bar: 50 μm. OT, optic tectum; CBV, cerebral blood vessel; HBV, hyaloid blood vessel; FI, fluorescence intensity.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Neurosci.