ZFIN ID: ZDB-FIG-121210-22
Shin et al., 2012 - Zebrafish neurofibromatosis type 1 genes have redundant functions in tumorigenesis and embryonic development. Disease models & mechanisms   5(6):881-894 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 2 Loss of nf1a and nf1b causes hyperplasia of OPCs and Schwann cells. (A,B) Confocal images of spinal cords in nf1a-/-; nf1b-/-; Tg(olig2:GFP) larvae (B) demonstrate increased numbers of dorsally migrating (white brackets) olig2:GFP-positive OPCs as compared with wild-type; Tg(olig2:GFP) larvae (A) at 4 dpf. (C,D) Confocal images of spinal cord in nf1a-/-; nf1b-/-; Tg(sox10:GFP) larvae (D) demonstrate increased numbers of both dorsally (white brackets) and ventrally (red brackets) positioned sox10:GFP-positive OPCs as compared with wild-type; Tg(sox10:GFP) larvae (C) at 4 dpf. (E,F) nf1a-/-; nf1b-/-; Tg(sox10:GFP) larvae (F) show an increased number of sox10:GFP-positive Schwann cells associated with the peripheral lateral line nerve (PLLn; white brackets) as compared with wild-type; Tg(sox10:GFP) larvae (E) at 4 dpf. (G,H) Neuronal numbers (olig2:GFP- [green], HuC/D- [magenta] positive) do not increase in concert with OPCs (olig2:GFP- [green], Sox10- [red] positive; arrowheads) in transverse sections through the spinal cord of nf1a/; nf1b-/-; Tg(olig2:GFP) larvae (H) as compared to wild-type; Tg(olig2:GFP) larvae (G) at 4 dpf. (I,J) Increased numbers of PLLn Schwann cells (sox10:GFP- [green], Sox10- [red] positive) are appreciated in transverse sections of nf1a-/-; nf1b-/-; Tg(sox10:GFP) larvae (J) as compared with wild-type; Tg(sox10:GFP) (I) larvae at 4 dpf. (K,L) Quantification of neurons (olig2:GFP-, HuC/D-positive cells) (K) and OPCs (olig2:GFP-, Sox10-positive cells) (L) from transverse sections through the spinal cord of wild-type; Tg(olig2:GFP) and nf1a-/-; nf1b-/-; Tg(olig2:GFP) larvae at 4 dpf. Values indicate mean + s.e.m. per section (n=30 from five each of wild-type and nf1a/; nf1b-/- larvae). (M) Quantification of sox10:GFP-positive Schwann cells in the PLLn of wild-type; Tg(sox10:GFP) and nf1a-/-; nf1b-/-; Tg(sox10:GFP) larvae at 4 dpf. Values indicate mean + s.e.m. per hemisegment (n=5 each for wild-type and nf1a-/-; nf1b-/- larvae). ***P<0.001. Scale bars: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ba2Tg standard conditions Day 4 posterior lateral line immature Schwann cell IHC
nf1ap301/p301; nf1bp303/p303; ba2Tg standard conditions Day 4 posterior lateral line immature Schwann cell IHC
nf1ap301/p301; nf1bp303/p303; vu12Tg standard conditions Day 4 spinal cord glioblast (sensu Vertebrata) IHC
Day 4 spinal cord neuron IHC
vu12Tg standard conditions Day 4 spinal cord glioblast (sensu Vertebrata) IHC
Day 4 spinal cord neuron IHC
sox10 Ab1-sox10 ba2Tg standard conditions Day 4 posterior lateral line immature Schwann cell IHC
Ab1-sox10 Day 4 spinal cord glioblast (sensu Vertebrata) IHC
Ab1-sox10 nf1ap301/p301; nf1bp303/p303; ba2Tg standard conditions Day 4 posterior lateral line immature Schwann cell IHC
Ab1-sox10 Day 4 spinal cord glioblast (sensu Vertebrata) IHC
Ab1-sox10 nf1ap301/p301; nf1bp303/p303; vu12Tg standard conditions Day 4 posterior lateral line immature Schwann cell IHC
Ab1-sox10 Day 4 spinal cord glioblast (sensu Vertebrata) IHC
Ab1-sox10 vu12Tg standard conditions Day 4 posterior lateral line immature Schwann cell IHC
Ab1-sox10 Day 4 spinal cord glioblast (sensu Vertebrata) IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC nf1ap301/p301; nf1bp303/p303; vu12Tg standard conditions Day 4 spinal cord neuron
IHC vu12Tg standard conditions Day 4 spinal cord neuron
Phenotype Details
Fish Conditions Stage Phenotype
nf1ap301/p301; nf1bp303/p303; ba2Tg standard conditions Day 4 posterior lateral line has extra parts of type immature Schwann cell, abnormal
nf1ap301/p301; nf1bp303/p303; vu12Tg standard conditions Day 4 spinal cord has extra parts of type glioblast (sensu Vertebrata), abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.