ZFIN ID: ZDB-FIG-121004-9
Jensen et al., 2012 - Opposing Effects of Circadian Clock Genes Bmal1 and Period2 in Regulation of VEGF-Dependent Angiogenesis in Developing Zebrafish. Cell Reports   2(2):231-241 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 4 Involvement of the Notch Signaling Pathway in Circadian Clock-Regulated Vascular Sprouting(A) Zebrafish embryos received bmal1 (B1MO, 0.2 pmol/embryo), period2 (P2MO, 0.2 pmol/embryo) or vegf (VEGFMO, 0.1 pmol/embryo) morpholinos, either alone or in combination with DAPT (100 μM), rbpsuh morpholino (RbpsuhMO, 0.1 pmol/embryo), or in the mind-bomb background. The embryos were subsequently raised under the LD condition. Arrows point to ISV braches in each group. Scale bar is 100 μm.(B) Quantification of the average ± SEM. ISV density or number of branches/segment of 72 hpf embryos in (A) (average n = 23 embryos/group).(C) Zebrafish embryos received period2 (P2MO, 0.2 pmol/embryo) alone or in combination with DAPT (100 μM), rbpsuh morpholino (RbpsuhMO, 0.1 pmol/embryo), or in the mind-bomb background. The embryos were subsequently raised under LD or LL conditions. Arrows point to ISV braches in each group. Scale bar is 100 μm.(D) Quantification of the average ± SEM. ISV density or number of branches/segment of 72 hpf embryos in (C) (average n = 23 embryos/group). p < 0.05; p < 0.001; and p < 0.001. NS, not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP mib1ta52b/ta52b; y1Tg control Protruding-mouth intersegmental vessel IFL
mib1ta52b/ta52b; y1Tg constant light Protruding-mouth intersegmental vessel IFL
mib1ta52b/ta52b; y1Tg + MO1-arntl1a standard conditions Protruding-mouth intersegmental vessel IFL
mib1ta52b/ta52b; y1Tg + MO1-per2 standard conditions Protruding-mouth intersegmental vessel IFL
mib1ta52b/ta52b; y1Tg + MO1-per2 constant light Protruding-mouth intersegmental vessel IFL
mib1ta52b/ta52b; y1Tg + MO1-vegfaa standard conditions Protruding-mouth intersegmental vessel IFL
y1Tg control Protruding-mouth intersegmental vessel IFL
y1Tg chemical treatment: DAPT Protruding-mouth intersegmental vessel IFL
y1Tg constant light Protruding-mouth intersegmental vessel IFL
y1Tg constant light, chemical treatment: DAPT Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-arntl1a chemical treatment: DAPT Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-arntl1a standard conditions Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-arntl1a + MO4-rbpja,rbpjb standard conditions Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-per2 chemical treatment: DAPT Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-per2 constant light, chemical treatment: DAPT Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-per2 standard conditions Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-per2 constant light Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-per2 + MO4-rbpja,rbpjb standard conditions Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-per2 + MO4-rbpja,rbpjb constant light Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-vegfaa chemical treatment: DAPT Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-vegfaa standard conditions Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-vegfaa + MO4-rbpja,rbpjb standard conditions Protruding-mouth intersegmental vessel IFL
y1Tg + MO4-rbpja,rbpjb constant light Protruding-mouth intersegmental vessel IFL
y1Tg + MO4-rbpja,rbpjb standard conditions Protruding-mouth intersegmental vessel IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mib1ta52b/ta52b; y1Tg control Protruding-mouth intersegmental vessel disorganized, abnormal
Protruding-mouth intersegmental vessel has extra parts of type blood vessel, abnormal
Protruding-mouth intersegmental vessel has extra parts of type vascular sprouts, abnormal
Protruding-mouth intersegmental vessel increased mass density, abnormal
y1Tg chemical treatment: DAPT Protruding-mouth intersegmental vessel disorganized, abnormal
Protruding-mouth intersegmental vessel has extra parts of type blood vessel, abnormal
Protruding-mouth intersegmental vessel has extra parts of type vascular sprouts, abnormal
Protruding-mouth intersegmental vessel increased mass density, abnormal
y1Tg + MO4-rbpja,rbpjb standard conditions Protruding-mouth intersegmental vessel disorganized, abnormal
Protruding-mouth intersegmental vessel has extra parts of type blood vessel, abnormal
Protruding-mouth intersegmental vessel has extra parts of type vascular sprouts, abnormal
Protruding-mouth intersegmental vessel increased mass density, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.