ZFIN ID: ZDB-FIG-120920-2
Constant Light Exposure Inhibits Developmental AngiogenesisTg(Fli1:EGFP)y1 transgenic zebrafish embryos exposed to LD (A–D), LL (A–D), or DD (C and D) were examined for ISV development at 24 hpf (C and D) or for SIV development at 72 hpf (C and D) using confocal microscopy.(A) Morphology of zebrafish embryos was examined at 24 hpf and 48 hpf under LD and LL conditions.(B) Average ± SEM somite lengths and head angles were quantified under LD and LL conditions (n = 7–9/group).(C) Zebrafish embryos were raised under LD, DD, or LL conditions from 1 hpf. Dashed lines indicate the maximal length of ISVs. Yellow dashed lines encircle the SIV. Scale bar for ISV is 50 μm and for SIV is 100 μm.(D) Quantification of the average ± SEM. ISV length (n = 52–54 embryos/group) of 24 hpf embryos and the SIV area of 72 hpf embryos (n = 48 embryos).(E) RT-PCR analysis of average zebrafish period2 (P2) expression ± SEM under LD, LL, and DD conditions (a pooled sample of 25 zebrafish embryos/group).(F) Densitometric quantification of average period2 expressions shown in (E).Bars in (A) = 500 μm. S, somite; NC, notochord; H, hindbrain; M, mesencephalon; E, eye. Blue lines in (A) indicate the head angle. p < 0.05 and p < 0.01. NS, not significant.See also Figure S2.
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