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ZIRC
ZFIN ID: ZDB-FIG-120920-2
Jensen et al., 2012 - Opposing Effects of Circadian Clock Genes Bmal1 and Period2 in Regulation of VEGF-Dependent Angiogenesis in Developing Zebrafish. Cell Reports   2(2):231-241 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 1 Constant Light Exposure Inhibits Developmental AngiogenesisTg(Fli1:EGFP)y1 transgenic zebrafish embryos exposed to LD (A–D), LL (A–D), or DD (C and D) were examined for ISV development at 24 hpf (C and D) or for SIV development at 72 hpf (C and D) using confocal microscopy.(A) Morphology of zebrafish embryos was examined at 24 hpf and 48 hpf under LD and LL conditions.(B) Average ± SEM somite lengths and head angles were quantified under LD and LL conditions (n = 7–9/group).(C) Zebrafish embryos were raised under LD, DD, or LL conditions from 1 hpf. Dashed lines indicate the maximal length of ISVs. Yellow dashed lines encircle the SIV. Scale bar for ISV is 50 μm and for SIV is 100 μm.(D) Quantification of the average ± SEM. ISV length (n = 52–54 embryos/group) of 24 hpf embryos and the SIV area of 72 hpf embryos (n = 48 embryos).(E) RT-PCR analysis of average zebrafish period2 (P2) expression ± SEM under LD, LL, and DD conditions (a pooled sample of 25 zebrafish embryos/group).(F) Densitometric quantification of average period2 expressions shown in (E).Bars in (A) = 500 μm. S, somite; NC, notochord; H, hindbrain; M, mesencephalon; E, eye. Blue lines in (A) indicate the head angle. p < 0.05 and p < 0.01. NS, not significant.See also Figure S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Protruding-mouth intersegmental vessel IFL
Protruding-mouth subintestinal vein IFL
y1Tg constant light Protruding-mouth intersegmental vessel IFL
Protruding-mouth subintestinal vein IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg constant light Protruding-mouth intersegmental vessel shortened, abnormal
Protruding-mouth subintestinal vein decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.