FIGURE

Fig. 8

ID
ZDB-FIG-120727-42
Publication
Niu et al., 2012 - Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 8

Sec13sq198 mutant protein is a C-terminal truncated Sec13 which is unable to interact with Sec31a. (A) Alignment of the C-terminal sequences of Sec13 and Sec13sq198. Sec13sq198 mutant protein lacks the C-terminus of Sec13 starting from D236 (blue arrow) and contains 34 new amino acids after Q235 (blue arrow). (B) Co-immunoprecipitation (Co-IP) analysis. HEK-293T cells were transfected with HA-SEC13 or HA-SEC13sq198 plasmids. An anti-HA antibody was used to immunoprecipitate (IP) the tagged proteins. The input (1st and 2nd panels) and the IP products (3rd and 4th panels) were blotted using an anti-SEC31A antibody (2nd and 4th panels) and an anti-Sec13 mosue serum (1st and 3rd panels). (C-E) In human Hela cells, Myc-SEC13 (C) or zebrafish Myc-Sec13 (D) co-localizes with endogenous SEC31a whilst SEC13sq198 is evenly distributed in the cytoplasm (E). (C1,D1,E1) Immunofluorescence staining of endogenous SEC31a (red). (C3,D3,E3) Immunostaining of Myc-tagged hSEC13 (C3) or zSec13 (D3) or hSEC13sq198 (E3) (green). (C2,D2,E2) Superimposed.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 367(2), Niu, X., Gao, C., Jan Lo, L., Luo, Y., Meng, C., Hong, J., Hong, W., and Peng, J., Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish, 197-207, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.