ZFIN Image: Niu et al., 2012, Fig. 8

Image

Figure Caption

Fig. 8 Sec13^sq198 mutant protein is a C-terminal truncated Sec13 which is unable to interact with Sec31a. (A) Alignment of the C-terminal sequences of Sec13 and Sec13^sq198. Sec13^sq198 mutant protein lacks the C-terminus of Sec13 starting from D²³⁶ (blue arrow) and contains 34 new amino acids after Q²³⁵ (blue arrow). (B) Co-immunoprecipitation (Co-IP) analysis. HEK-293T cells were transfected with HA-SEC13 or HA-SEC13^sq198 plasmids. An anti-HA antibody was used to immunoprecipitate (IP) the tagged proteins. The input (1st and 2nd panels) and the IP products (3rd and 4th panels) were blotted using an anti-SEC31A antibody (2nd and 4th panels) and an anti-Sec13 mosue serum (1st and 3rd panels). (C-E) In human Hela cells, Myc-SEC13 (C) or zebrafish Myc-Sec13 (D) co-localizes with endogenous SEC31a whilst SEC13^sq198 is evenly distributed in the cytoplasm (E). (C1,D1,E1) Immunofluorescence staining of endogenous SEC31a (red). (C3,D3,E3) Immunostaining of Myc-tagged hSEC13 (C3) or zSec13 (D3) or hSEC13^sq198 (E3) (green). (C2,D2,E2) Superimposed.

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Reprinted from Developmental Biology, 367(2), Niu, X., Gao, C., Jan Lo, L., Luo, Y., Meng, C., Hong, J., Hong, W., and Peng, J., Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish, 197-207, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.