FIGURE

Fig. S5

ID

ZDB-FIG-120725-46

Publication

Vasilyev et al., 2012 - Mechanical Stretch and PI3K Signaling Link Cell Migration and Proliferation to Coordinate Epithelial Tubule Morphogenesis in the Zebrafish Pronephros

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Fig. S5

Inhibiting CDK4/CyclinD1 signaling phenocopies the effects of inhibiting PI3K, related to Figure 3. Inhibiting CDK4/CyclinD1 using 20 μM NSC625987 results in exaggerated linear stretching of the distal tubule (A), ET11-9 transgenic. This chemical treatment also resulted in significant reduction in distal kidney BrdU incorporation (B), P<0.05. (C): the segment lengths were affected by NSC625987 treatment in a manner very similar to LY294002. The proximal tubule becomes significantly longer (P<0.01), the collecting duct becomes significantly shorter (P<0.01). Unlike the LY294002 result, the distal tubule is slightly shorter in NSC625987 treatment condition. This small, but statistically significant effect (P<0.05) could be due to small direct effect of NSC625987 on the migration rate (distal tubule elongation is migration dependent).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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