header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-120710-56
Delous et al., 2012 - sox9b Is a Key Regulator of Pancreaticobiliary Ductal System Development. PLoS Genetics   8(6):e1002754 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 7 The pattern of Notch signaling activity is altered in sox9b mutants.

(A–F) Expression of Tg(Tp1bglob:H2B-mCherry) (red), 2F11 (blue), and Tg(Tp1bglob:VenusPest) (green) in wild-type (A–C) and sox9b mutant (D–F) larvae at 75 (A, D), 99 (B, E), and 123 hpf (C, F). At each time point, 6 larvae of each genotype were analyzed. (A2–F2) Diagrams showing the distribution of Tg(Tp1bglob:H2B-mCherry);Tg(Tp1bglob:VenusPest)-double positive cells (yellow) and Tg(Tp1bglob:H2B-mCherry)-single positive cells (red) in (A–F). Livers are outlined by solid white line. In wild-type livers, expression of Tg(Tp1bglob:H2B-mCherry) and Tg(Tp1bglob:VenusPest) largely overlaps at 75 hpf (A, A′). At 99 and 123 hpf (B–B′, C–C′), a few Tg(Tp1bglob:H2B-mCherry)-single positive cells (arrows) appear along the intrahepatic duct that connects to the extrahepatic system. In sox9b mutants, Tg(Tp1bglob:H2B-mCherry);Tg(Tp1bglob:VenusPest)-double positive cells and Tg(Tp1bglob:H2B-mCherry)-single positive cells show similar distribution as in wild-type at 75 and 99 hpf (D–D′, E–E′). However, at 123 hpf (F, F′), we observed big clusters of Tg(Tp1bglob:H2B-mCherry)-single positive cells in the mutant livers (arrows). (G) Percentages (average±SEM) of Tg(Tp1bglob:H2B-mCherry)-single positive cells relative to the total number of Tg(Tp1bglob:H2B-mCherry)-expressing cells. Whereas wild-type and sox9b heterozygous livers contained similar percentages of Tg(Tp1bglob:H2B-mCherry)-single positive cells at all stages examined (p>0.4), this percentage was significantly higher in sox9b mutants at 123 hpf (p<0.0005). (H) Percentages (average±SEM) of Tg(Tp1bglob:H2B-mCherry)-single positive cells or Tg(Tp1bglob:H2B-mCherry);Tg(Tp1bglob:VenusPest)-double positive cells that were labeled by EdU. EdU incubation was conducted from 96 to 120 hpf or from 120 to 148 hpf. Under both conditions, the hepatic Notch responsive cells in sox9b mutants showed higher EdU incorporation compared to wild-type, and the difference was more pronounced for Tg(Tp1bglob:H2B-mCherry)-single positive cells. 7 wild-types and 7 sox9b mutants were examined for each experimental condition. Asterisks indicate statistical significance: *, p<0.05. (A–F) All images are projections of confocal z-stacks. Ventral views, anterior (A) to the top. Scale bar, 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry s939Tg; s940Tg standard conditions Protruding-mouth to Day 5 liver IFL
sox9bfh313/fh313; s939Tg; s940Tg standard conditions Protruding-mouth to Day 5 liver IFL
Venus s939Tg; s940Tg standard conditions Protruding-mouth to Day 5 liver IFL
sox9bfh313/fh313; s939Tg; s940Tg standard conditions Protruding-mouth to Day 5 liver IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sox9bfh313/fh313; s939Tg; s940Tg standard conditions Day 5 Notch signaling pathway disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.