ZFIN ID: ZDB-FIG-120525-44
Coolen et al., 2012 - miR-9 Controls the Timing of Neurogenesis through the Direct Inhibition of Antagonistic Factors. Developmental Cell   22(5):1052-1064 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 3

miR-9 Knockdown Delays Cell-Cycle Exit, Leading to the Amplification of Late-Born Neuronal Populations (A) Scheme illustrating the experimental procedure. (B) Transverse sections showing immunohistochemical detection of BrdU (purple) and GFP in the HuC:GFP line in a pulse chase experiment between 30 and 48 hpf. (C) Comparison of the number of BrdU-positive cells that are also HuC-GFP-positive (green bars) or not (purple bars), or the total number of BrdU-positive cells per section (gray and black bars) between control (light colors) and morphant embryos (dark colors) (n = 7). (D) Expression of barhl2 in control and miR-9 MO injected embryos at 48 hpf. The left panels are lateral views, the middle panels are dorsal views, and the right panels are higher magnification of the latter, in the anterior hindbrain. (E) Number of Barhl2-positive cells per 5-μm section at 48 hpf (n = 5). p < 0.01. Values are presented as mean ± SEM. See also Figure S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
barhl2 WT control Long-pec hindbrain neuron ISH
WT + MO1-mir9 standard conditions Long-pec hindbrain neuron ISH
EGFP knu3Tg control Long-pec hindbrain neuron IHC
knu3Tg + MO1-mir9 standard conditions Long-pec hindbrain neuron IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-mir9 standard conditions Long-pec hindbrain exit from mitosis delayed, abnormal
Long-pec hindbrain lateral region has extra parts of type neuron, abnormal
Long-pec hindbrain neurogenesis increased duration, abnormal
Long-pec hindbrain neurogenesis increased occurrence, abnormal
knu3Tg + MO1-mir9 standard conditions Long-pec hindbrain lateral region has extra parts of type neuron, abnormal
Long-pec hindbrain neurogenesis increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 22(5), Coolen, M., Thieffry, D., Drivenes, O., Becker, T.S., and Bally-Cuif, L., miR-9 Controls the Timing of Neurogenesis through the Direct Inhibition of Antagonistic Factors, 1052-1064, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell