FIGURE

Fig. 4

ID
ZDB-FIG-120425-6
Publication
Tobin et al., 2012 - Host genotype-specific therapies can optimize the inflammatory response to mycobacterial infections
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Modulation of TNF Levels Results in Genotype-Specific Rescue of LTA4H-Mediated Hypersusceptibility

(A) Mean (±SEM) number of bacteria per infected macrophage 40-44 hpi with 150-200 erp mutant M. marinum of wild-type or TNF morphant (MO) siblings.

(B) FPC in control or tnf morphant siblings 3 dpi with 90-100 M. marinum.

(C) Quantitation of frequency of bacterial cording of the animals in (B).

(D) Quantitation of neutral red positive cells 4 dpi after infection with 100 M. marinum in sibling controls or tnf morphants.

(E) Mean (±SEM) number of bacteria per infected macrophage at 40-44 hpi in wild-type animals with or without injection of 0.5 ng rTNF 12 hr after infection with 150-200 erp mutant M. marinum.

(F) FPC in control or rTNF injected siblings 3 dpi with 90-100 M. marinum.

(G) Quantitation of frequency of bacterial cording of the animals in (F).

(H) Quantitation of neutral red positive cells at 4 dpi after infection with 90-100 M. marinum in sibling controls or rTNF injected animals.

(I) FPC in wild-type or lta4h morphant siblings 3 dpi with 90-100 M. marinum per animal after injection of 0.5 ng rTNF at 12 hpi.

(J) Bacterial cording frequency of the animals in (I).

(K) FPC in wild-type animals, LTA4H-high siblings, and LTA4H-high plus TNF morphant animals, at 3 dpi with 90-100 M. marinum.

(L) Quantitation of frequency of bacterial cording in the animals in (K).

Statistical comparisons in panels (I) and (K) by one-way ANOVA with Tukey′s post hoc test; in panels (C),(G),(J) and (L) by Fisher′s exact test; (A),(B),(D),(E),(F),(H) by Student′s unpaired t test. For all panels p < 0.05; p < 0.01; p < 0.001, all other comparisons not significant. See also Figure S3.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 148(3), Tobin, D.M., Roca, F.J., Oh, S.F., McFarland, R., Vickery, T.W., Ray, J.P., Ko, D.C., Zou, Y., Bang, N.D., Chau, T.T., Vary, J.C., Hawn, T.R., Dunstan, S.J., Farrar, J.J., Thwaites, G.E., King, M.C., Serhan, C.N., and Ramakrishnan, L., Host genotype-specific therapies can optimize the inflammatory response to mycobacterial infections, 434-446, Copyright (2012) with permission from Elsevier. Full text @ Cell