ZFIN ID: ZDB-FIG-120328-6
Zhao et al., 2012 - Kinesin-2 family in vertebrate ciliogenesis. Proc. Natl. Acad. Sci. USA   109(7):2388-2393 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Adult

Fig. 3

kif17 mutant phenotype. (A) Upper: Sequences of WT and kif17sa0119 mutant allele. Lower: Diagram of the Kif17 protein. Arrow indicates the site of the stop codon. (B) Upper: Quantitative RT-PCR. Expression level relative to WT is provided. Data were normalized for actin expression (P < 0.05). Lower: RT-PCR amplification of the kif17 transcript in WT and mutant embryos at 5 dpf. (C and C′) External phenotype of WT (C) and kif17sa0119 (C′) larvae at 7 dpf. (D) Confocal and ultrastructural analysis of cilia in WT and kif17sa0119 mutants. Larvae were stained with anti-acetylated tubulin antibody to visualize cilia in the nasal pit (a and d) and ear cristae (b and e). c and f, Ultrathin sections perpendicular to the distal tips of olfactory cilia in WT and mutant, respectively. (E) Graph showing the length distribution of nasal cilia in WT and kif17sa0119 mutants at 7 dpf. Each dot represents the average cilia length measured in a single individual. “n” is the number of individuals analyzed (P ≤ 0.001). (F) Confocal images of transverse cryosections through the retina of adult WT (a and c) and kif17 homozygous mutant (b and d) individuals stained with Zpr-1 (a and b) or anti-rod opsin (c and d) antibodies (green) and counterstained with phalloidin (red). Arrowheads indicate the outer limiting membrane, asterisks the outer plexiform layer. (G) Measurement of opsin transport efficiency from the inner to the outer segment in WT and kif17sa0119 mutant animals at 4 dpf. The intensity of GFP–opsin signal in the cell body is measured at 4, 9, and 24 h after heat shock. For each data point, 15 to 20 retinae, and 30 to 36 photoreceptors were analyzed. This experiment was performed as published (29) and illustrated in Fig. S1 (*P < 0.05; **P < 0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
rho WT standard conditions Adult retinal rod cell IHC
kif17sa0119/sa0119 standard conditions Adult retinal rod cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC WT standard conditions Adult outer limiting membrane
IHC Adult retinal photoreceptor layer
IHC kif17sa0119/sa0119 standard conditions Adult outer limiting membrane
IHC Adult retinal photoreceptor layer
Phenotype Details
Fish Conditions Stage Phenotype
kif17sa0119/sa0119 standard conditions Day 5 retinal cone cell development process quality, normal
kif17sa0119/sa0119 standard conditions Day 5 retinal cone cell photoreceptor inner segment morphology, normal
kif17sa0119/sa0119 standard conditions Day 5 retinal rod cell photoreceptor inner segment morphology, normal
kif17sa0119/sa0119 standard conditions Day 5 whole organism morphology, normal
kif17sa0119/sa0119 standard conditions Days 7-13 lateral crista cilium morphology, normal
kif17sa0119/sa0119 standard conditions Days 7-13 olfactory epithelium cilium decreased length, abnormal
kif17sa0119/sa0119 standard conditions Days 7-13 olfactory epithelium motile cilium present, normal
kif17sa0119/sa0119 standard conditions Adult retinal cone cell development process quality, normal
kif17sa0119/sa0119 standard conditions Adult retinal cone cell photoreceptor inner segment morphology, normal
kif17sa0119/sa0119 standard conditions Adult retinal rod cell photoreceptor inner segment morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Proc. Natl. Acad. Sci. USA for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA