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ZFIN ID: ZDB-FIG-120315-57
Petrey et al., 2012 - Excessive activity of cathepsin K is associated with the cartilage defects in a zebrafish model for mucolipidosis II. Disease models & mechanisms   5(2):177-190 Full text @ Dis. Model. Mech.
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Day 4

Fig. 5

Cathepsin expression is enriched in the craniofacial skeleton of zebrafish embryos. (A–C) Analysis of enzyme activities in lysates of 3-dpf WT and ML-II embryos separated into heads and tails, as diagrammed in A (dashed line represents cut site) demonstrates that cathepsin activities are primarily increased in the head in ML-II embryos compared with WT (B) rather than in the tail (C); n=3. (D–G) In situ hybridization for cathepsin K expression shows mRNA enrichment in ventral tissues that generally correspond to the pharyngeal skeleton at 2 dpf (D,E) and 3 dpf (F,G) in WT and ML-II embryos. Arrows denote differences in the pattern of expression between WT and ML-II embryos. CB, ceratobranchials; M, Meckels cartilage. (H–J) Immunohistochemical stains for cathepsin K (red) on sections of 3 dpf (H) and 4 dpf (I,J) WT fli1a:EGFP embryos show that it is expressed in chondrocytes and peri-chondrial fibroblasts of the trabecular (H,J) and Meckels cartilages (I).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctsk WT standard conditions Long-pec splanchnocranium ISH
Protruding-mouth splanchnocranium ISH
Ab1-ctsk y1Tg control Protruding-mouth Meckel's cartilage chondrocyte IHC
Ab1-ctsk Protruding-mouth Meckel's cartilage perichondrium IHC
Ab1-ctsk Day 4 Meckel's cartilage chondrocyte IHC
Ab1-ctsk Day 4 Meckel's cartilage perichondrium IHC
y1Tg + MO2-gnptab standard conditions Long-pec splanchnocranium ISH
Protruding-mouth splanchnocranium ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.