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General Information
ZIRC
ZFIN ID: ZDB-FIG-120301-5
van Eeden et al., 1996 - Mutations affecting somite formation and patterning in the zebrafish, Danio rerio. Development (Cambridge, England)   123:153-164
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to 20-25 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 14-19 somites to 20-25 somites

Fig. 9 (A) Engrailed (4D9) staining of a 21-somite stage wild-type embryo. Staining is visible at the midbrain-hindbrain boundary and in the somites. In every somite about 2-5 nuclei, representing the muscle pioneers, are stained. In a yot mutant at the same stage (B), staining of the muscle pioneers is absent. (C) 4D9 staining of a wildtype sibling and (D) the weakest you-type mutant, youtm146c. Approximately one Engrailed-positive cell is present per segment. (E-G) myoD-staining of a wild type, a yot mutant and a putative yot/+ embryo at the 17-somite stage, respectively. In the homozygous yot mutant, adaxial staining is absent and the staining in the rest of the paraxial mesoderm is reduced (G). In the yot heterozygous embryo, expression in the adaxial cells is reduced, especially in the presomitic mesoderm, anterior to the tailbud (F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myod1 WT standard conditions 14-19 somites adaxial cell ISH
gli2aty119/+ standard conditions 14-19 somites adaxial cell ISH
gli2aty119/ty119 standard conditions 14-19 somites Not Detected adaxial cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-eng IHC WT standard conditions 20-25 somites midbrain hindbrain boundary
IHC 20-25 somites muscle pioneer nucleus
IHC 20-25 somites somite muscle pioneer
IHC gli2aty119/ty119 standard conditions 20-25 somites midbrain hindbrain boundary
IHC 20-25 somites Not Detected somite muscle pioneer
IHC scube2tm146c/tm146c standard conditions 20-25 somites Not Detected muscle pioneer nucleus
IHC 20-25 somites Not Detected somite muscle pioneer
Phenotype Details
Fish Conditions Stage Phenotype
gli2aty119/+ standard conditions 14-19 somites adaxial cell decreased amount, abnormal
gli2aty119/ty119 standard conditions 14-19 somites adaxial cell absent, abnormal
20-25 somites somite lacks all parts of type muscle pioneer, abnormal
scube2tm146c/tm146c standard conditions 20-25 somites somite has fewer parts of type muscle pioneer, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.