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ZFIN ID: ZDB-FIG-120228-25
Nicoli et al., 2012 - miR-221 Is Required for Endothelial Tip Cell Behaviors during Vascular Development. Developmental Cell   22(2):418-429 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 2

miR-221 Is Required for Vegfc/Flt4 Signaling (A–F) Confocal micrographs Tg(fli1a:egfp)y1 embryos at 27 hpf; arrowheads denote ISVs; lateral views, dorsal is up, anterior to the left. (A) Embryo injected with 6 ng control MO. (B) Embryo injected with 1 ng flt4 and 5 ng control MO. (C) Embryo injected with 1 ng control and 5 ng miR-221 MO. (D) Embryo injected with 1 ng flt4 MO and 5 ng miR-221 MO. (E) kdrlum19 mutant embryo injected with 10 ng control MO. (F) kdrlum19 mutant embryo injected with 10 ng of miR-221 MO. (G) Quantification of ISV length in wild-type or kdrlum19 mutant embryos injected with indicated MOs. Measurements were made of four adjacent ISVs per embryo in at least 18 embryos from three separate injections; -p < 0.001; N.S., not statistically different. (H–K) Tg(fli1a:egfp)y1 embryos injected with hsp70:vegfc-2A-mcherry transposable element and heat shocked at 15 ss. Red indicates Vegfc-2A-mcherry expression. Arrowheads indicate ectopic formation of vessels. Embryos at 33 hpf coinjected with (H) 10 ng control MO, (I) 2 ng flt4 MO, (J) 10 ng miR-221 MO, or (K) mutant for kdrlum19. (L) Proportion of hemisegments with ectopic vessel sprouts in embryos injected with 25 pg Tol2-hsp70:vegfc-2A-mcherry and 25 pg Tol2 transposase. Embryos were co-injected with indicated MO, or were mutant for the kdrlum19 allele as determined by genotyping following phenotypic analysis. -p < 0.02; N.S., not significant. Scale bars are (A–F) 50 µm and (H–K) 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s916Tg; y7Tg standard conditions Prim-15 intersegmental vessel IHC
s916Tg; y7Tg + MO1-mir221 standard conditions Prim-15 intersegmental vessel IHC
y1Tg standard conditions Prim-15 intersegmental vessel IHC
y1Tg + MO1-flt4 standard conditions Prim-15 intersegmental vessel IHC
y1Tg + MO1-flt4 + MO1-mir221 standard conditions Prim-15 intersegmental vessel IHC
y1Tg + MO1-mir221 standard conditions Prim-15 intersegmental vessel IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s916Tg; y7Tg standard conditions Prim-15 intersegmental vessel decreased length, abnormal
s916Tg; y7Tg + MO1-mir221 standard conditions Prim-15 intersegmental vessel decreased length, normal
y1Tg + MO1-flt4 standard conditions Prim-15 intersegmental vessel decreased length, normal
y1Tg + MO1-flt4 + MO1-mir221 standard conditions Prim-15 intersegmental vessel decreased length, abnormal
y1Tg + MO1-mir221 standard conditions Prim-15 intersegmental vessel decreased length, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 22(2), Nicoli, S., Knyphausen, C.P., Zhu, L.J., Lakshmanan, A., and Lawson, N.D., miR-221 Is Required for Endothelial Tip Cell Behaviors during Vascular Development, 418-429, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell