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ZFIN ID: ZDB-FIG-111102-2
Gutnick et al., 2011 - The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary. Developmental Cell   21(4):642-654 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Days 7-13

Fig. 2

Axovasal Interactions in the Zebrafish Pituitary

(A–D) Three-dimensional reconstructions of the hypophysis of 9-day-old zebrafish embryos carrying pairs of transgenic fluorescent markers of different hypophyseal components.

(A) The oxtl:EGFP transgene (green) marks oxytocinergic axons terminating at the neurohypophysis, just dorsal and posterior to the adenohypophyseal prolactin-producing cells marked with prl:RFP (red).

(B) Double transgenic animals expressing oxtl:EGFP (green) together with the vascular endothelial marker vegfr2:Cherry (red) reveal a distinct, previously unidentified structure of the zebrafish hypophyseal vasculature.

(C and D) Hypophyseal vasculature, visualized by either vegfr2:EGFP or vegfr2:Cherry, together with the adenohypophyseal markers prl:RFP and pomc:EGFP. The ring-like vascular structure resided dorsal to the adenohypophysis. (B2) and (D2) show optical Z-slices that demonstrate the position of the NH in an indentation of the adenohypophysis.

(E) Schematic map of ventral head vasculature of the zebrafish larvae, including the location of the hypophyseal vasculature. The asterisk marks the location of the neurohypophysis.

AH, adenohypophysis; CrDI, cranial division of the internal carotid artery; NH, neurohypophysis; OA, optic artery; PLA, palatocerebral artery; PLV, palatocerebral vein; pomc, proopiomelanocortin; prl, prolactin; vegfr, vascular endothelial growth factor receptor. Scale bar, 20 μm. See also mmc3VIDEO, mmc4VIDEO and mmc5VIDEO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s843Tg; zf113Tg standard conditions Days 7-13 hypophysis vascular endothelium IHC
s896Tg; wz01Tg standard conditions Days 7-13 neurohypophysis axon terminus IHC
wz01Tg; zf113Tg standard conditions Days 7-13 neurohypophysis axon terminus IHC
GFP s896Tg; zf44Tg standard conditions Days 7-13 adrenocorticotropic hormone secreting cell IHC
mCherry s896Tg; wz01Tg standard conditions Days 7-13 hypophysis vascular endothelium IHC
s896Tg; zf44Tg standard conditions Days 7-13 hypophysis vascular endothelium IHC
RFP s843Tg; zf113Tg standard conditions Days 7-13 prolactin secreting cell IHC
wz01Tg; zf113Tg standard conditions Days 7-13 prolactin secreting cell IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 21(4), Gutnick, A., Blechman, J., Kaslin, J., Herwig, L., Belting, H.G., Affolter, M., Bonkowsky, J.L., and Levkowitz, G., The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary, 642-654, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell