ZFIN ID: ZDB-PUB-111027-47
The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary
Gutnick, A., Blechman, J., Kaslin, J., Herwig, L., Belting, H.G., Affolter, M., Bonkowsky, J.L., and Levkowitz, G.
Date: 2011
Source: Developmental Cell 21(4): 642-654 (Journal)
Registered Authors: Affolter, Markus, Belting, Heinz-Georg Paul (Henry), Blechman, Janna, Bonkowsky, Joshua, Gutnick, Amos, Kaslin, Jan, Levkowitz, Gil
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cells, Cultured
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Endothelium, Vascular/cytology
  • Endothelium, Vascular/drug effects*
  • Endothelium, Vascular/metabolism
  • Gene Expression Regulation, Developmental/drug effects
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Hypothalamo-Hypophyseal System/cytology
  • Hypothalamo-Hypophyseal System/drug effects
  • Hypothalamo-Hypophyseal System/metabolism
  • Hypothalamus/drug effects*
  • Hypothalamus/growth & development
  • Hypothalamus/metabolism
  • Immunoenzyme Techniques
  • Neurons/cytology
  • Neurons/drug effects*
  • Neurons/metabolism
  • Oxytocics/pharmacology
  • Oxytocin/pharmacology*
  • Pituitary Gland/blood supply*
  • Pituitary Gland/cytology*
  • Pituitary Gland/drug effects
  • Zebrafish/embryology
PubMed: 22014522 Full text @ Dev. Cell
FIGURES
ABSTRACT
The hypothalamo-neurohypophyseal system (HNS) is the neurovascular structure through which the hypothalamic neuropeptides oxytocin and arginine-vasopressin exit the brain into the bloodstream, where they go on to affect peripheral physiology. Here, we investigate the molecular cues that regulate the neurovascular contact between hypothalamic axons and neurohypophyseal capillaries of the zebrafish. We developed a transgenic system in which both hypothalamic axons and neurohypophyseal vasculature can be analyzed in vivo. We identified the cellular organization of the zebrafish HNS as well as the dynamic processes that contribute to formation of the HNS neurovascular interface. We show that formation of this interface is regulated during development by local release of oxytocin, which affects endothelial morphogenesis. This cell communication process is essential for the establishment of a tight axovasal interface between the neurons and blood vessels of the HNS. We present a unique example of axons affecting endothelial morphogenesis through secretion of a neuropeptide.
ADDITIONAL INFORMATIONNo data available