ZFIN ID: ZDB-FIG-110914-28
Shono et al., 2011 - Acquisition of glial cells missing 2 Enhancers Contributes to a Diversity of Ionocytes in Zebrafish. PLoS One   6(8):e23746 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 3

gcm2 is essential for the development of HRCs and suppresses the development of NCCCs on the skin surface.

(A, B) Whole-mount in situ hybridization of zebrafish 48 hours post-fertilization (hpf) with an atp6v1al probe as a HRC marker. (C, D) Whole-mount in situ hybridization with an atp1b1b probe as a NaRC marker. (E, F) Whole-mount in situ hybridization with a slc12a10.2 probe as a NCCC marker. (A, C, E) Zebrafish (48 hpf) injected with a control morpholino (MO). (B, D, F) Zebrafish (48 hpf) injected with a gcm2 MO. (G) Quantitative comparison of atp6v1al-positive cells, atp1b1b-positive cells, and slc12a10.2-positive cells in zebrafish injected with control MO and gcm2 MO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atp1b1b WT standard conditions Long-pec NaK ionocyte ISH
WT + MO4-gcm2 standard conditions Long-pec NaK ionocyte ISH
atp6v1aa WT standard conditions Long-pec vH ionocyte ISH
WT + MO4-gcm2 standard conditions Long-pec vH ionocyte ISH
slc12a10.2 WT standard conditions Long-pec NCC ionocyte ISH
WT + MO4-gcm2 standard conditions Long-pec NCC ionocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-gcm2 standard conditions Long-pec NCC ionocyte increased amount, abnormal
Long-pec vH ionocyte decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One