ZFIN ID: ZDB-FIG-110622-89
Yang et al., 2011 - BMP and non-canonical Wnt signaling are required for inhibition of secondary tail formation in zebrafish. Development (Cambridge, England)   138(12):2601-2611 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 5

N-cadherin is required to maintain tailbud integrity. (A,B) Percentages of bifurcated tail in zebrafish embryos injected with the indicated combination of morpholinos (MOs). For low concentrations, dvl2/3 (1 mg/ml each), mfn (0.5 mg/ml) and chd2 (0.1 mg/ml) MOs were injected. For each column over 100 embryos from three separate experiments were scored. (C-G) Lateral view of tails of embryos at 24 hpf injected with the indicated combination of morpholinos, after in situ hybridization for ntl. ntl expression in cdh2 and dvl2/3 MO morphants (C,D) is similar to wild type. Note that co-injection of a high concentration of chd2 and dvl2/3 MOs further enhanced the ntl expression from a single fork shaped pattern (E) to a multi-fork pattern (F), but co-injection of high concentration of chd2 and mfn MOs (G) did not result in a similar phenotype. This difference is consistent with the following antibody staining results (see below). For high concentrations, dvl2/3 (1.5 mg/ml each), mfn (1.5 mg/ml) and chd2 (0.25 mg/ml) MOs were injected. (H-J) Representative confocal microscopy images of tailbud in wild-type, kny and mfn embryos stained with pan-cadherin antibody at 21 somites; inset, close-up of the caudal end of notochord. Membrane staining pattern in the caudal end of notochord is shown in the wild type (H) and the mfn (J) mutant, whereas diffused staining pattern is shown in the kny mutant (I).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tbxta WT standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
WT + MO1-cdh2 standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
WT + MO1-cdh2 + MO1-dvl2 + MO1-dvl3a standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
WT + MO1-cdh2 + MO2-tll1 standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pan-Cadherin IHC WT standard conditions 20-25 somites notochord posterior region plasma membrane
IHC 20-25 somites tail bud
IHC gpc4fr6/fr6 standard conditions 20-25 somites tail bud
IHC tll1tc263a/tc263a standard conditions 20-25 somites notochord posterior region plasma membrane
IHC 20-25 somites tail bud
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cdh2 standard conditions Prim-5 post-vent region morphology, normal
WT + MO1-cdh2 + MO1-dvl2 + MO1-dvl3a standard conditions Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
WT + MO1-cdh2 + MO2-tll1 standard conditions Prim-5 notochord morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-anal tail morphogenesis having extra processual parts post-anal tail morphogenesis, abnormal
Prim-5 post-vent region deformed, abnormal
Prim-5 whole organism has extra parts of type post-vent region, abnormal
gpc4fr6/fr6 standard conditions 20-25 somites notochord morphogenesis disrupted, abnormal
gpc4fr6/fr6 standard conditions 20-25 somites notochord posterior region cell separated from notochord posterior region cell, abnormal
tll1tc263a/tc263a standard conditions 20-25 somites notochord morphogenesis disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development