ZFIN ID: ZDB-FIG-110621-17
Langenbacher et al., 2011 - The PAF1 complex differentially regulates cardiomyocyte specification. Developmental Biology   353(1):19-28 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5

Fig. s3

Morpholinos targeting PAF1C component genes reduce target protein levels. (A–D) Western blots detecting PAF1C component proteins and β-actin protein levels in uninjected control and morpholino injected embryo lysates. (A) Ctr9 protein is reduced to 38% of control levels in ctr9MO injected embryos. (B) Paf1 protein is reduced to 43% of control levels in paf1MO injected embryos. (C) Cdc73 protein is reduced to 39% of control levels in cdc73MO injected embryos. (D) Rtf1 protein is reduced to 7% of control levels in rtf1MO injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdc73 Ab1-cdc73 WT standard conditions Prim-5 whole organism WB
Ab1-cdc73 WT + MO2-cdc73 standard conditions Prim-5 whole organism WB
ctr9 Ab1-ctr9 WT standard conditions Prim-5 whole organism WB
Ab1-ctr9 WT + MO1-ctr9 standard conditions Prim-5 whole organism WB
paf1 Ab1-paf1 WT standard conditions Prim-5 whole organism WB
Ab1-paf1 WT + MO2-paf1 standard conditions Prim-5 whole organism WB
rtf1 Ab1-rtf1 WT standard conditions Prim-5 whole organism WB
Ab1-rtf1 WT + MO1-rtf1 standard conditions Prim-5 whole organism WB
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 353(1), Langenbacher, A.D., Nguyen, C.T., Cavanaugh, A.M., Huang, J., Lu, F., and Chen, J.N., The PAF1 complex differentially regulates cardiomyocyte specification, 19-28, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.