ZFIN ID: ZDB-FIG-110408-5
Corti et al., 2011 - Interaction between alk1 and blood flow in the development of arteriovenous malformations. Development (Cambridge, England)   138(8):1573-1582 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Prim-25

Fig. 5

Vasodilation contributes to increased vessel caliber in alk1 mutants. (A) Qtracker 655 non-targeted quantum dots were injected into the common cardinal vein of wild-type and alk1 mutant Tg(fli1a:nEGFP)y7 embryos. Images are two-dimensional confocal projections, dorsal views, anterior leftwards. Embryos aged 32-36 hpf are shown. Endothelial cell nuclei are green; quantum dots are magenta. Scale bar: 50 μm. Endothelial cell density in the BCA (white arrowhead) was calculated from these micrographs by dividing the number of nuclei by the vessel volume. Data represent meanĀ±s.e.m. (n=5-7 independent samples) and were analyzed using Student′s t-test, **P<0.001. (B) Analysis of the contribution of edn1 loss and cxcr4a upregulation to AVM development. AVM development in alk1-/- embryos (asterisk) is not phenocopied by edn1-/- embryos even in the presence of heterozygous levels of alk1 (edn1-/-;alk+/-). cxcr4a-/- embryos do not exhibit AVMs, whereas cxcr4a-/-;alk1+/- embryos invariably develop AVMs, demonstrating that cxcr4a upregulation is not necessary for AVM development in alk1 mutants. Two-dimensional confocal projections of Tg(fli1a:EGFP)y1;Tg(gata1:dsRed)sd2 or Tg(kdrl:GFP)la116;Tg(gata1:dsRed)sd2 embryos. Endothelial cells are green; erythrocytes are magenta. Dorsal views, anterior leftwards. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed acvrl1y6/+; edn1tf216b/tf216b; la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
acvrl1y6/y6; cxcr4aum20/um20; la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
acvrl1y6/y6; la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
cxcr4aum20/um20; la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
edn1tf216b/tf216b; la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
EGFP acvrl1y6/+; edn1tf216b/tf216b; la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
acvrl1y6/y6; cxcr4aum20/um20; la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
acvrl1y6/y6; la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
cxcr4aum20/um20; la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
edn1tf216b/tf216b; la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
acvrl1y6/+; edn1tf216b/tf216b; la116Tg; sd2Tg standard conditions Prim-15 cranial vasculature morphology, normal
acvrl1y6/y6; cxcr4aum20/um20; la116Tg; sd2Tg standard conditions Prim-25 basal communicating artery attached to primordial midbrain channel, abnormal
Prim-25 basilar artery attached to primordial hindbrain channel, abnormal
acvrl1y6/y6; y7Tg standard conditions Prim-15 basal communicating artery endothelial cell decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development