ZFIN ID: ZDB-FIG-110408-5
Corti et al., 2011 - Interaction between alk1 and blood flow in the development of arteriovenous malformations. Development (Cambridge, England)   138(8):1573-1582 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Prim-25

Fig. 5

Vasodilation contributes to increased vessel caliber in alk1 mutants. (A) Qtracker 655 non-targeted quantum dots were injected into the common cardinal vein of wild-type and alk1 mutant Tg(fli1a:nEGFP)y7 embryos. Images are two-dimensional confocal projections, dorsal views, anterior leftwards. Embryos aged 32-36 hpf are shown. Endothelial cell nuclei are green; quantum dots are magenta. Scale bar: 50 μm. Endothelial cell density in the BCA (white arrowhead) was calculated from these micrographs by dividing the number of nuclei by the vessel volume. Data represent meanĀ±s.e.m. (n=5-7 independent samples) and were analyzed using Student′s t-test, **P<0.001. (B) Analysis of the contribution of edn1 loss and cxcr4a upregulation to AVM development. AVM development in alk1-/- embryos (asterisk) is not phenocopied by edn1-/- embryos even in the presence of heterozygous levels of alk1 (edn1-/-;alk+/-). cxcr4a-/- embryos do not exhibit AVMs, whereas cxcr4a-/-;alk1+/- embryos invariably develop AVMs, demonstrating that cxcr4a upregulation is not necessary for AVM development in alk1 mutants. Two-dimensional confocal projections of Tg(fli1a:EGFP)y1;Tg(gata1:dsRed)sd2 or Tg(kdrl:GFP)la116;Tg(gata1:dsRed)sd2 embryos. Endothelial cells are green; erythrocytes are magenta. Dorsal views, anterior leftwards. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed acvrl1y6/+; edn1tf216b/tf216b; la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
acvrl1y6/y6; cxcr4aum20/um20; la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
acvrl1y6/y6; la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
cxcr4aum20/um20; la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
edn1tf216b/tf216b; la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
la116Tg; sd2Tg standard conditions Prim-25 nucleate erythrocyte IFL
EGFP acvrl1y6/+; edn1tf216b/tf216b; la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
acvrl1y6/y6; cxcr4aum20/um20; la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
acvrl1y6/y6; la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
cxcr4aum20/um20; la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
edn1tf216b/tf216b; la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
la116Tg; sd2Tg standard conditions Prim-25 endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
acvrl1y6/+; edn1tf216b/tf216b; la116Tg; sd2Tg standard conditions Prim-15 cranial vasculature morphology, normal
acvrl1y6/y6; cxcr4aum20/um20; la116Tg; sd2Tg standard conditions Prim-25 basal communicating artery attached to primordial midbrain channel, abnormal
Prim-25 basilar artery attached to primordial hindbrain channel, abnormal
acvrl1y6/y6; y7Tg standard conditions Prim-15 basal communicating artery endothelial cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development