ZFIN ID: ZDB-FIG-110408-1
Corti et al., 2011 - Interaction between alk1 and blood flow in the development of arteriovenous malformations. Development (Cambridge, England)   138(8):1573-1582 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Prim-25

Fig. 1

Blood flow modifies endothelial cell number in alk1 mutants. (A) Wiring diagrams of perfused head vessels in 48 hpf wild-type and alk1-/- embryos, derived from two-dimensional confocal projections of Tg(gata1:dsRed)sd2 embryos. Arteries are red; veins are blue. alk1 mutants exhibit enlarged arteries and AVMs (*) between the BCA/PMBC and/or BA/PHBC. Vessels represented in wild-type embryos but not alk1-/- embryos are present but not patent in mutants. AA1, first aortic arch; BA, basilar artery; BCA, basal communicating artery; CaDI, caudal division of internal carotid artery; ICA, internal carotid artery; LDA, lateral dorsal aorta; PCS, posterior communicating segments; PHBC, primordial hindbrain channel; PMBC, primordial midbrain channel. Scale bar: 50 μm. (B) Development of the BCA/PCSs in wild type (row 1), alk1-/- (row 2), tnnt2a morphants (MO) (row 3) and alk1-/-;tnnt2a MO (row 4). Images are two-dimensional confocal projections of live Tg(fli1a:nEGFP)y7;Tg(fli1a.ep:mRFP-F)pt505 embryos, dorsal views, anterior leftwards. Endothelial cell nuclei are green; endothelial cell membranes are magenta. In alk1-/-, the BCA (arrowhead) is enlarged by 36 hpf, and the PCSs (arrows) by 40 hpf. BCA/PCS morphology is relatively normal in tnnt2 MO and alk1-/-;tnnt2 MO, although vessels are collapsed. Scale bar: 50 μm. (C) Quantification of endothelial cell number from confocal micrographs of fixed Tg(fli1a:nEGFP)y7 embryos. alk1-/- with (black bars) or without (green bars) blood flow show similar increases in endothelial cell number compared with wild-type siblings (white bars) between 32-40 hpf, although the pronounced increase between 40 and 48 hpf observed in alk1-/- depends on blood flow. Cell number in tnnt2a MO (blue bars) is not different from cell number in alk1-/-;tnnt2a MO, suggesting that lack of flow phenocopies alk1 mutants in terms of endothelial cell number. Data represent mean±s.e.m. (n=3-13 independent samples) and were analyzed by Student′s t-test. *P<0.01; **P<0.001. (D) Quantification of endothelial cell number from confocal micrographs of 50 hpf wild-type or alk1 MO Tg(fli1a:nEGFP)y7 embryos treated with tricaine between 42 and 50 hpf. The increase in endothelial cell number observed at 40-48 hpf in alk1 morphants is reduced by stopping blood flow. Data represent mean±s.e.m. (n=4-8 independent samples) and were analyzed by Student′s t-test. **P<0.001

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP acvrl1y6/y6; pt505Tg; y7Tg standard conditions Prim-25 endothelial cell nucleus IFL
Long-pec endothelial cell nucleus IFL
acvrl1y6/y6; pt505Tg; y7Tg + MO1-tnnt2a standard conditions Prim-25 endothelial cell nucleus IFL
Long-pec endothelial cell nucleus IFL
pt505Tg; y7Tg standard conditions Prim-25 endothelial cell nucleus IFL
Long-pec endothelial cell nucleus IFL
pt505Tg; y7Tg + MO1-tnnt2a standard conditions Prim-25 endothelial cell nucleus IFL
Long-pec endothelial cell nucleus IFL
RFP acvrl1y6/y6; pt505Tg; y7Tg standard conditions Prim-25 endothelial cell IFL
Long-pec endothelial cell IFL
acvrl1y6/y6; pt505Tg; y7Tg + MO1-tnnt2a standard conditions Prim-25 endothelial cell IFL
Long-pec endothelial cell IFL
pt505Tg; y7Tg standard conditions Prim-25 endothelial cell IFL
Long-pec endothelial cell IFL
pt505Tg; y7Tg + MO1-tnnt2a standard conditions Prim-25 endothelial cell IFL
Long-pec endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
acvrl1y6/y6 standard conditions Prim-15 basal communicating artery endothelial cell increased amount, abnormal
Prim-15 posterior communicating artery endothelial cell increased amount, abnormal
acvrl1y6/y6; pt505Tg; y7Tg standard conditions Prim-25 basal communicating artery increased size, abnormal
Prim-25 posterior communicating artery increased size, abnormal
acvrl1y6/y6; pt505Tg; y7Tg + MO1-tnnt2a standard conditions Prim-25 blood vessel collapsed, abnormal
pt505Tg; y7Tg + MO1-tnnt2a standard conditions Prim-25 blood vessel collapsed, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development