ZFIN ID: ZDB-FIG-101122-44
Sarmah et al., 2010 - Inositol hexakisphosphate kinase-2 acts as an effector of the vertebrate Hedgehog pathway. Proceedings of the National Academy of Sciences of the United States of America   107(46):19921-19926 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Day 5

Fig. 1 IP6K2 activity is required for normal development of craniofacial and somite structures. (A–C) Lateral view of uninjected (A), ip6k2SPMO-injected (B), and ip6k2SPMO + ip6k2 mRNA-coinjected (C) live embryos at 24 hpf. Eighty percent of the ip6k2SPMO embryos had a small head and reduced trunk; both the defects were rescued by ip6k2 mRNA coinjection. Effective ip6k2SPMO suppression of splicing was confirmed by RT-PCR (Fig. S3 A and B). (D–K) Ventral view of Alcian blue stained head skeleton of 5-d-old uninjected control (D), ip6k2SPMO (E–G), ip6k2SPMO + ip6k2 mRNA (H), ipk2SPMO (I), and ipk1MO1 (J and K)-injected embryos. The anterior neurocranium was mostly maintained in 40% of ip6k2MO embryos, but distorted or reduced in 60% of embryos (F and G). The pharyngeal skeleton was affected in 92% embryos injected (n = 90). ip6k2 mRNA coinjection restored normal craniofacial skeleton (H). Craniofacial skeleton was altered in ipk2SPMO embryos (I). No craniofacial deformity was evident in ipk1MO1 embryos (J and K). e, eye; m, Meckel′s cartilage; pq, palatoquadrate; ch, ceratohyal; cb 3–7, ceratobranchial arches 3–7; eth, ethmoid plate.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ipmkb standard conditions Day 5 cranium hypoplastic, abnormal
Day 5 embryonic viscerocranium morphogenesis disrupted, abnormal
WT + MO1-ippk standard conditions Day 5 determination of heart left/right asymmetry disrupted, abnormal
WT + MO3-ip6k2a standard conditions Prim-5 head decreased size, abnormal
Prim-5 somite development disrupted, abnormal
Prim-5 somite U-shaped, abnormal
Prim-5 trunk decreased size, abnormal
Day 5 cranial cartilage hypoplastic, abnormal
Day 5 embryonic viscerocranium morphogenesis disrupted, abnormal
Day 5 Meckel's cartilage decreased length, abnormal
Day 5 neurocranium anterior region decreased size, abnormal
Day 5 neurocranium anterior region deformed, abnormal
Day 5 palatoquadrate cartilage decreased length, abnormal
Day 5 pharyngeal arch 2 decreased length, abnormal
Day 5 pharyngeal arch cartilage morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA